We narrowed to 1,102 results for: cas12a

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6xHis-MBP-huCMtCas12a

Plasmid

#174680

Purpose

Expresses 6xHis-MBP tagged CMtCas12a

Depositor

Piyush Jain

Publication

Nguyen et al medRxiv 2021.07.21

Insert

huCMtCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKEW107-MbCas12a-33362

Plasmid

#115654

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR control

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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LbCas12a-DR-GC-swap-cr in pBCSK+

Plasmid

#126643

Purpose

Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Publication

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat GC Swap crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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FnoCas12a-DR-cr in PBCSK-

Plasmid

#126642

Purpose

Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Publication

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHS641_Flex-Cas12a-ABE

Plasmid

#244846

Purpose

Mammalian expression of Flex-Cas12a adenine base editor

Depositor

Jennifer Doudna

Publication

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-dCas12a-ABE

Use

CRISPR

Tags

SV40 NLS-ABE8e

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q/D832A

Promoter

CMV

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pUPD2 LbCas12a (GB1439)

Plasmid

#160573

Purpose

Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

LbCas12a

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 AsCas12a (GB1438)

Plasmid

#160572

Purpose

Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AsCas12a

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

Plasmid

#160611

Purpose

Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:LbCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKEW321-MbCas12a-RFP-GFP-array

Plasmid

#115661

Purpose

Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

GFP and RFP array for MbCas12

Use

CRISPR

Expression

Bacterial

Promoter

Plac

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJCC_058 FnCas12a D917A

Plasmid

#171667

Purpose

For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Publication

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a D917A

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Mutation

changed aspartate 917 to alanine

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-PxCas12a-2AeGFP

Plasmid

#123637

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

PxCas12a

Tags

3xHA tag

Expression

Mammalian

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-ArCas12a-2AeGFP

Plasmid

#123632

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

ArCas12a

Tags

3xHA tag

Expression

Mammalian

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-BsCas12a-2AeGFP

Plasmid

#123633

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

BsCas12a

Tags

3xHA tag

Expression

Mammalian

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-LpCas12a-2AeGFP

Plasmid

#123635

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

LpCas12a

Tags

3xHA tag

Expression

Mammalian

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCH62 (denAsCas12a-KRAB piggyBac)

Plasmid

#217333

Purpose

piggyBac expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

D908A/E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-LbCas12a

Plasmid

#213743

Purpose

For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

Depositor

Caixia Gao

Publication

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ289 (ErCas12a)

Plasmid

#138126

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

ErCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracr_AsCas12a.DR

Plasmid

#155050

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Publication

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)

Use

CRISPR

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Topo_SpCas9.tracr_LbCas12a.DR

Plasmid

#155049

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Publication

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)

Use

CRISPR

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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