We narrowed to 6,397 results for: eed

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pBabe.puro.CDK8.KD.flag

Plasmid

#19759

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

D173A (kinase dead)

Available Since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only
pBabe.puro.CDK8.KD.flag

Plasmid

#19759

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

D173A (kinase dead)

Available Since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only
hU6-pegRNA-EFS-mScarlet Template

Plasmid

#196037

Purpose

The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Insert

mScarlet

Expression

Mammalian

Promoter

hU6 and EFS

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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hU6-pegRNA-EFS-mScarlet Template

Plasmid

#196037

Purpose

The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Insert

mScarlet

Expression

Mammalian

Promoter

hU6 and EFS

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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EFS-SpdCas9-Dnmt3A/3L-WT

Plasmid

#222497

Purpose

Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

Depositor

Isaac Hilton

Article

Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

Insert

S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion

Use

Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

EFS

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-SpdCas9-Dnmt3A/3L-WT

Plasmid

#222497

Purpose

Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

Depositor

Isaac Hilton

Article

Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

Insert

S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion

Use

Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

EFS

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

Plasmid

#210710

Purpose

This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/N580A

Promoter

M11

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

Plasmid

#210710

Purpose

This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/N580A

Promoter

M11

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1.shCDK8

Plasmid

#19760

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against CDK8 (CDK8 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pLKO.1.shCDK8

Plasmid

#19760

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against CDK8 (CDK8 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pBS-EF1-IRES-NEO-Control

Plasmid

#80757

Purpose

Transposon Reporter

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

NeoR (neoR Synthetic)

Promoter

EF1-IRES from PB-EF1-IRES-NEO

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-EF1-IRES-NEO-Control

Plasmid

#80757

Purpose

Transposon Reporter

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

NeoR (neoR Synthetic)

Promoter

EF1-IRES from PB-EF1-IRES-NEO

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG1b_10xHisTEVATG5_ATG12

Plasmid

#190861

Purpose

Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG12 (ATG12 Human)

Tags

6xHis-TEV

Expression

Bacterial and Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBIG1b_10xHisTEVATG5_ATG12

Plasmid

#190861

Purpose

Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG12 (ATG12 Human)

Tags

6xHis-TEV

Expression

Bacterial and Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHR ErbB2-CD3ε-FusionRed

Plasmid

#188628

Purpose

Human ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

ERBB2 (ERBB2 Synthetic, Human)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHR ErbB2-CD3ε-FusionRed

Plasmid

#188628

Purpose

Human ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

ERBB2 (ERBB2 Synthetic, Human)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB3-v1

Plasmid

#154893

Purpose

Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB3 (ERBB3 Human)

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB3-v1

Plasmid

#154893

Purpose

Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB3 (ERBB3 Human)

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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LV-AR-AAFF

Plasmid

#171218

Purpose

Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

Depositor

Steven Balk

Article

Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

Insert

AR-AAFF (AR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

AR-K580A-V581A

Promoter

EF-1a

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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LV-AR-AAFF

Plasmid

#171218

Purpose

Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

Depositor

Steven Balk

Article

Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

Insert

AR-AAFF (AR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

AR-K580A-V581A

Promoter

EF-1a

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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