Showing: 1 - 20 of 1570 results
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5TTW (EED)
Plasmid#101254PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEED (EED Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
EED-F97A
Plasmid#131326PurposegRNA to generate F97A cage-mutant EEDDepositorInsertEED-KO-gRNA-2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAS-8 (seed1-8)
Plasmid#40843PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–seed1-8
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailabilityAcademic Institutions and Nonprofits only -
pSMP-EED_2
Plasmid#36386DepositorInsertEED (EED Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMVHA EED wt
Plasmid#24231PurposeMammalian expression of human EED with HA tagDepositorInsertEED (EED Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EED
Plasmid#125163PurposeExpresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.EED
Plasmid#125174PurposeExpresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
EED-KO-gRNA-1
Plasmid#131321PurposegRNA to knockout EED. Use with EED-KO-gRNA-2DepositorInsertEED-KO-gRNA-1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
EED-KO-gRNA-2
Plasmid#131322PurposegRNA to knockout EED. Use with EED-KO-gRNA-1DepositorInsertEED-KO-gRNA-2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
shTRIP13-2-seed
Plasmid#184535PurposeshRNA expression vectorDepositorInsertTRIP13 (TRIP13 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
siCheck2-4xmiR307_23nt-seedonly
Plasmid#41100DepositorInsert4xmiR307_23nt-seedonly
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
siCheck2-4xmiR307_21nt-seedonly
Plasmid#41101DepositorInsert4xmiR307_21nt-seedonly
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
siCheck2-8xmiR307_23nt-seedonly
Plasmid#41102DepositorInsert8xmiR307_23nt-seedonly
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
siCheck2-8xmiR307_21nt-seedonly
Plasmid#41103DepositorInsert8xmiR307_21nt-seedonly
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-TuDmiR122 seedonly
Plasmid#35667DepositorInsertTuD miR-122seedonly
UseTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-TuDmiR122 seedsupplemental
Plasmid#35668DepositorInsertTuD miR-122seedsupplemental
UseTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-YFP-Eed
Plasmid#82512PurposeLentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.DepositorInsertembryonic ectoderm development
UseLentiviralTagsHaloTagExpressionMammalianMutationinsert contains AA39-441 of EeDPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 1570 results