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5TTW (EED)
Plasmid#101254PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEED (EED Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
EED-F97A
Plasmid#131326PurposegRNA to generate F97A cage-mutant EEDDepositorInsertEED-KO-gRNA-2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEED
Plasmid#219658PurposePermits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)DepositorInsertEmpty SEED Cassette (3xP3-dsRed)
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWG_RUBYseed
Plasmid#225989PurposeTo clone any gene of interest using gateway recombination cloning and the vector expresses seed-specific RUBYDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pSEED-mGreenLantern:ALFA
Plasmid#219644PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest methodDepositorInsertmGreenLantern:ALFA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-sfGFP
Plasmid#219639PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mScarlet3
Plasmid#219640PurposePermits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest methodDepositorInsertmScarlet SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-Dendra2
Plasmid#219645PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest methodDepositorInsertDendra2 SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA EED wt
Plasmid#24231PurposeMammalian expression of human EED with HA tagDepositorInsertEED (EED Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSEED-OLLAS:HA
Plasmid#219647PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest methodDepositorInsertOLLAS:HA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-sfGFP:ALFA
Plasmid#219642PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest methodDepositorInsertsfGFP:ALFA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mGreenLantern
Plasmid#219643PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest methodDepositorInsertmGreenLantern SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EED
Plasmid#125163PurposeExpresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mCherry
Plasmid#219657PurposePermits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.EED
Plasmid#125174PurposeExpresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EED_2
Plasmid#36386DepositorInsertEED (EED Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAS-8 (seed1-8)
Plasmid#40843PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–seed1-8
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only
