...Guidelines for giving feedback Use formal and informal mechanisms for feedback (more on this below). ...management style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to...well. Find out the Effect of the Feedback: How does this feedback compare with your own perception? ...What help did you need? What help did you solicit? When did you first see you needed help? What kept...the way to go when giving feedback. The employee is not helped if the feedback session is over and they...a 5 part series on Management for scientists. Feedback is help and it should result in authentic assistance...effective and successful. Giving timely and useful feedback is an absolutely required aspect of being a successful...

...that brings feedback back to the manager. Team members rarely rush to give honest feedback to the manager...these); ask for feedback and input on multiple topics every time you meet.  Seek feedback in multiple formats...changes influenced by feedback. If you don't do this, employees will stop providing feedback pretty quickly....communication especially important for a manager – getting feedback from the team. Successful management can almost...to learn about her management style.   Seeking feedback frequently One of the most important paths of ... variety of reasons. They may feel that giving feedback will be perceived as complaining. They may worry...successful manager that is genuinely receptive to feedback and so receives this valuable input from at least...

...busy scientist working hard on research, and you need the right experimental materials. Who has time for...

...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research...

...mice can be included in breeding strategies identical to those used for breeding heterozygous mice. In ...all of the offspring in the F1 generation, your breeding scheme should start with parental mice that are...detect your targeting vector or gene of interest. 2. Breed your parental mice to generate F1 Once you’ve performed...when hemizygous, meaning that only one chromosome needs to contain the transgene in order to see an effect...locus (e.g. Cre) and heterozygous at the other. 3. Breed your F1 generation Now, your goal is to generate...as progeny from future generations. 4. Continue breeding your colony You’ll want to generate enough mice...harbor similar epigenetic modifications. Special breeding cases Whether your mouse colony consists of genetically...

...at the cost of flexibility to address the unique needs of students from different departments across the...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014...

...recognize that storing data in a sharable form is indeed a significant effort. Our standards of data recording...

...step out there into the galaxy. TL;DR: Many roads need scientists open for adventure. Many thanks to our...

...consensus on genome editing, there was a generally agreed-upon international moratorium on utilizing CRISPR...academic conference response to He created a positive feedback loop that rewarded unethical behavior (it’s hard...

... few months ago, Addgene has been working with Seeding Labs to provide plasmid grants to researchers in...awards has successfully concluded, Addgene and Seeding Labs will be opening applications for a second ...researchers who have received equipment through Seeding Labs’ Instrumental Access program. We are excited... Resources Apply to Recieve Equipment through Seeding Labs' Instrumental Access Program Additional Resources... seeding labs...

... can get involved with Seeding Labs Follow Seeding Labs on Twitter @SeedingLabs or on Facebook Additional... that need to face these challenges locally. We look forward to more collaborations with Seeding Labs ... proud to announce that we are teaming up with Seeding Labs to better serve those in developing countries...Developing an international scientific community Seeding Labs, a nonprofit founded in 2007, strives to expand...Through its flagship program, Instrumental Access, Seeding Labs partners with donors to supply surplus lab...and talent is just a first step; going forward, Seeding Labs hopes to expand its activities by facilitating...more to Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important...

...influence the level of transgene expression, and may need to be optimized for each unique application (Trapani...Riyad JM, Weber T (2016) Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus...

...will run for at least 6 months as we assess the needs of the scientific community and possibly expand ...

...hard to appreciate without computational support. Indeed, rational sgRNA design is not possible without ...was subjectively ascribed relative to the basic needs of each individual; the  community as a whole has... are trying to solve, and finally how we might proceed in the future. When we think of designing experiments...collaborative effort from the community in order to succeed. With respect to de novo sgRNA design, I again ...you to select the tool(s) based on your project needs. There are 8 major categories found in the table...Benchling. Utility functions: These functions help speed up the sgRNA design process by removing repetitive...Different communities develop software for their own needs. This often results in software design that is applicable...

... the shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative...More About the Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees... the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists .... We are excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress....to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and promote the awards...

...Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding Labs Plasmid Grant Awardees...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to these researchers...researchers. In the third round of the Addgene-Seeding Labs plasmid grant we are excited to introduce ...modify the course of this condition are urgently needed. Therefore, understanding neuronal networks, their.... We are excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress....to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and promote the awards...

...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD2-dU6:2gRNA dU6...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD3-dU6:3gRNA dU6...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD4-U6:1_U6:3tandemgRNAs...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pAc-sgRNA-Cas9 dU6...

...transcription none, need Cas9 plasmid Joung pMB70 cU6 BsaI Transfection none, need Cas9 plasmid Boxem ...transcription none, need Cas9 plasmid Boxem PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...-scaffold SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg L4440_BioBrick-sgRNA T7 BbsI... BbsI, BsaI Ingestion by C. elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids...

...spacers into the PX330 vector backbone? There is no need to add the NGG PAM sequence. Because the 'NGG' of...the PAM is used to select your genomic target, you need to make sure the NGG immediately follows your target... order is: 5'-CACCGN...N-3' 3'-CN...NCAAA-5' You need to add the CACC and AAAC cloning overhang. When ...starts with a 'G', you naturally have one and do not need to add an additional 'G'. Where are double strand...be sufficient to generate off-target DSBs; there needs to be extensive homology of the protospacer followed...your cells. If you want to use double nickase, you need to have four spacers co-expressed and using PX335...the double nickase system, with PX335 vector, you need to find two guides for each cleavage site, but this...