CRISPR Plasmids - Drosophila
Type
Collection
...type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA...you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target... or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD2-dU6:2gRNA dU6...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD3-dU6:3gRNA dU6...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD4-U6:1_U6:3tandemgRNAs...Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pAc-sgRNA-Cas9 dU6...