...Guidelines for giving feedback Use formal and informal mechanisms for feedback (more on this below). ...management style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to...well. Find out the Effect of the Feedback: How does this feedback compare with your own perception? ...What help did you need? What help did you solicit? When did you first see you needed help? What kept...the way to go when giving feedback. The employee is not helped if the feedback session is over and they...a 5 part series on Management for scientists. Feedback is help and it should result in authentic assistance...effective and successful. Giving timely and useful feedback is an absolutely required aspect of being a successful...

...that brings feedback back to the manager. Team members rarely rush to give honest feedback to the manager...these); ask for feedback and input on multiple topics every time you meet.  Seek feedback in multiple formats...changes influenced by feedback. If you don't do this, employees will stop providing feedback pretty quickly....communication especially important for a manager – getting feedback from the team. Successful management can almost...to learn about her management style.   Seeking feedback frequently One of the most important paths of ... variety of reasons. They may feel that giving feedback will be perceived as complaining. They may worry...successful manager that is genuinely receptive to feedback and so receives this valuable input from at least...

...busy scientist working hard on research, and you need the right experimental materials. Who has time for...

...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research...

...mice can be included in breeding strategies identical to those used for breeding heterozygous mice. In ...all of the offspring in the F1 generation, your breeding scheme should start with parental mice that are...detect your targeting vector or gene of interest. 2. Breed your parental mice to generate F1 Once you’ve performed...when hemizygous, meaning that only one chromosome needs to contain the transgene in order to see an effect...locus (e.g. Cre) and heterozygous at the other. 3. Breed your F1 generation Now, your goal is to generate...as progeny from future generations. 4. Continue breeding your colony You’ll want to generate enough mice...harbor similar epigenetic modifications. Special breeding cases Whether your mouse colony consists of genetically...

...at the cost of flexibility to address the unique needs of students from different departments across the...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014...

...recognize that storing data in a sharable form is indeed a significant effort. Our standards of data recording...

...step out there into the galaxy. TL;DR: Many roads need scientists open for adventure. Many thanks to our...

...consensus on genome editing, there was a generally agreed-upon international moratorium on utilizing CRISPR...academic conference response to He created a positive feedback loop that rewarded unethical behavior (it’s hard...

... few months ago, Addgene has been working with Seeding Labs to provide plasmid grants to researchers in...awards has successfully concluded, Addgene and Seeding Labs will be opening applications for a second ...researchers who have received equipment through Seeding Labs’ Instrumental Access program. We are excited... Resources Apply to Recieve Equipment through Seeding Labs' Instrumental Access Program Additional Resources... seeding labs...

... can get involved with Seeding Labs Follow Seeding Labs on Twitter @SeedingLabs or on Facebook Additional... that need to face these challenges locally. We look forward to more collaborations with Seeding Labs ... proud to announce that we are teaming up with Seeding Labs to better serve those in developing countries...Developing an international scientific community Seeding Labs, a nonprofit founded in 2007, strives to expand...Through its flagship program, Instrumental Access, Seeding Labs partners with donors to supply surplus lab...and talent is just a first step; going forward, Seeding Labs hopes to expand its activities by facilitating...more to Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important...

...influence the level of transgene expression, and may need to be optimized for each unique application (Trapani...Riyad JM, Weber T (2016) Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus...

...will run for at least 6 months as we assess the needs of the scientific community and possibly expand ...

...hard to appreciate without computational support. Indeed, rational sgRNA design is not possible without ...was subjectively ascribed relative to the basic needs of each individual; the  community as a whole has... are trying to solve, and finally how we might proceed in the future. When we think of designing experiments...collaborative effort from the community in order to succeed. With respect to de novo sgRNA design, I again ...you to select the tool(s) based on your project needs. There are 8 major categories found in the table...Benchling. Utility functions: These functions help speed up the sgRNA design process by removing repetitive...Different communities develop software for their own needs. This often results in software design that is applicable...

... the shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative...More About the Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees... the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists .... We are excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress....to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and promote the awards...

...Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding Labs Plasmid Grant Awardees...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to these researchers...researchers. In the third round of the Addgene-Seeding Labs plasmid grant we are excited to introduce ...modify the course of this condition are urgently needed. Therefore, understanding neuronal networks, their.... We are excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress....to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and promote the awards...

...will need compensation? To know if you will need to compensate in your flow panel, you will need to know...would say that APC is “bleeding,” or spilling over, into APC-Cy7 channel and needs to be compensated out...this bleed. A compensation value of 3.7 for APC into APC-Cy7 applied next, which fixed the bleed in this...fluorescent markers are read by the same laser, they will need to be compensated for one another. This overlap ...fluorophores FITC and PE on the same laser, you will need to compensate between the two of them because they...reads them out on different lasers, you should not need to compensate between them. Another rule of thumb...of the fluorescent markers are similar, you will need to compensate between them (although there are exceptions...

...Identify the skills needed to use these tools Outline the knowledge and skill sets needed to do your science...that you’ll need to tap for your future research team. For example, if you’re going to need a tech because...Experiment Designs Consulting, Inc. “How much do you need [for your lab start up]?” A hiring chair or dean...doesn’t have to be this way, if you know what you need. Most often, early career scientists aren’t trained...instead have our clients focus on their experimental needs and consider a budget as a resource checklist. A... budget is an account of the material resources needed to accomplish your science, and can include: equipment...discussion first defines the material resources needed, then determines how much those resources will ...

... germline Selecting for seeds that have the tDNA insertions (usually via seed growth on antibiotic-containing...fungal growth. Healthy plants produce lots of seeds. More seeds mean more chances to recover a transformant...of time to produce seeds after you expose the flowers to Agrobacterium. More seeds mean more chances to...produce lots of seeds. I typically use 8-10 plants per construct I transform. More seeds mean more chances... transformants based on expression of RFP in the seed (Emami, Yee and Dinneny, 2013). Preparing plants...transformation efficiency. If you see many mature siliques (seed pods), your plants may be too old to transform. ...mature prior to transformation are just full of seeds that you know won’t harbor your transgene. You can...