Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 32,631 results for: ple

Showing: 2581 - 2600 of 32631 results

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pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

Plasmid

#204213

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on I207M

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204211

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on Q175* (*termination)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204210

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on R178* (*termination)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

Plasmid

#204212

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NK2 homeobox 1 (NKX2-1 Human)

Use

Lentiviral

Tags

Point mutation on I207F

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

Plasmid

#204724

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166134

Purpose

This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting firefly luciferase gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

Plasmid

#167199

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R S364L

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

Plasmid

#167200

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R D421N

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

Plasmid

#167198

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R E294K

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

Plasmid

#167197

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R M173I

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

Plasmid

#167196

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R E144K

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

Plasmid

#130678

Purpose

Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D409_F489del

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

Plasmid

#130691

Purpose

Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

E653_G1960del

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

Plasmid

#130690

Purpose

Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, G1720_Y1846del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

Plasmid

#138147

Purpose

Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

Plasmid

#167935

Purpose

Inducible CRISPRi expression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

KRAB-dCas9-DHFR

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

Plasmid

#138150

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

Plasmid

#138148

Purpose

Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NES

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

Plasmid

#106171

Purpose

cre mediated red to green shift

Depositor

Ewa Snaar-Jagalska

Article

Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

Inserts

dsRED-express2
eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

Plasmid

#167937

Purpose

Inducible CRISPRa expression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

dxCas9(3.7)-VPR

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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Showing: 2581 - 2600 of 32631 results

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