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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,963 results for: pcDNA3.1+

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Showing: 2601 - 2620 of 6963 results

  1. pHS1862

    Plasmid
    #205975
    Purpose
    FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas5 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205975-plasmid-map-sequence-id-416239

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  2. pHS1859

    Plasmid
    #205972
    Purpose
    FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205972-plasmid-map-sequence-id-416242

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  3. pHS1860

    Plasmid
    #205973
    Purpose
    FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205973-plasmid-map-sequence-id-416241

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  7. pcDNA-GFP-EphrinB3-Flag(A227Y)

    Plasmid
    #155013
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EphrinB3 (EPHB3 Human)
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    A227Y
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155013-plasmid-map-sequence-id-310418

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  8. pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

    Plasmid
    #216491
    Purpose
    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells
    Depositor
    Jin Zhang
    Publication
    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloAKAR2.1
    Tags
    NES, T2A-EGFP-CAAX
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    216491-plasmid-map-sequence-id-431039

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  11. pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

    Plasmid
    #169992
    Purpose
    Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation
    Depositor
    Daniel Bachovchin
    Publication
    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CARD8 (CARD8 Human)
    Tags
    TEV-siriusGFP-FLAG
    Expression
    Mammalian
    Mutation
    S297A
    Promoter
    CMV
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    169992-plasmid-map-sequence-id-335591

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  15. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid
    #163991
    Purpose
    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging
    Depositor
    Hao Wu
    Publication
    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLRP1 CARD (NLRP1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    163991-plasmid-map-sequence-id-324596

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  16. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid
    #126444
    Purpose
    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated
    Depositor
    Jeremy Wilusz
    Publication
    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:
    Insert
    Fndc3b (Fndc3b Mouse)
    Expression
    Mammalian
    Mutation
    circFndc3b exons are separated by a miniature int…
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126444-plasmid-map-sequence-id-245088

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  19. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

    Plasmid
    #53551
    Purpose
    mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"
    Depositor
    Kathleen Collins
    Publication
    Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPP1 (ACD Human)
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    contains aa 88-544, with aa 166-172 replaced wit…
    Promoter
    CMV
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid
    #50888
    Purpose
    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50888-plasmid-map-sequence-id-76361

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Showing: 2601 - 2620 of 6963 results