We narrowed to 10,319 results for: poly

1. pLVX-Flag-Polß(L301R/V306R)-IRES-puro

   Plasmid

   #128656

   Purpose

   mammalian expression of Flag-Polß(L301R/V306R) with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L301R/V306R

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Flag-Polß(KTM)-IRES-puro

   Plasmid

   #128672

   Purpose

   mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K41R/K61R/K81R

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-Flag-Polß(K206A/K244A)-IRES-puro

   Plasmid

   #128683

   Purpose

   mammalian expression of Flag-Polß(K206A/K244A) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K206A/K244A

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Flag-Polß(TM-K244A)-IRES-puro

   Plasmid

   #128680

   Purpose

   mammalian expression of Flag-Polß(TM-K244A: L301R/V303R/V306R-K244A) portein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L301R/V303R/V306R/K244A

   Promoter

   CMV

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-Flag-Polß(TM)-IRES-neo

   Plasmid

   #128671

   Purpose

   mammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein with neomycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L301R/V303R/V306R

   Promoter

   CMV

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

   Plasmid

   #99690

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

   Plasmid

   #99694

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1953

   Plasmid

   #142897

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV uN2C GFP (Ctl)

   Plasmid

   #224355

   Purpose

   Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

   Insert

   uN2C
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Codon-optimized for expression in human, so no pu…

   Promoter

   CMV + chimeric intyron (CAG)

   Available since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P90C

    Plasmid

    #114606

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 42 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P80C

    Plasmid

    #114602

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P80C cysteine mutations and a 22 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-A60C

    Plasmid

    #114601

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 22 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, A…

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P70C

    Plasmid

    #114605

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P70C cysteine mutations and a 42 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-A60C

    Plasmid

    #114604

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 42 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, A…

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P90C

    Plasmid

    #114603

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 22 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AF20-double-tag

    Plasmid

    #108389

    Purpose

    Express human PC1

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-eGFP-PC1-3HA

    Plasmid

    #108406

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    3HA and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-mPC1_4077-eGFP-3HA

    Plasmid

    #108390

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-mPC1_4119-3HA

    Plasmid

    #108395

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-Flag-Polß(TM-KTM)-IRES-puro

    Plasmid

    #128676

    Purpose

    mammalian expression of Flag-Polß(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R/-K41R/K61R/K81R

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge