We narrowed to 11,622 results for: Vars

1. pDL026_HA-rb_IgG_LC_15F11

   Plasmid

   #193623

   Purpose

   HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-HA_15F11 rabbit light chain
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pBR322-TIMER

   Plasmid

   #103056

   Purpose

   medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC_N_KLF9

   Plasmid

   #167774

   Purpose

   Expresses KLF9 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   KLF9 (KLF9 Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiGuide

   Plasmid

   #117986

   Purpose

   pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

   Depositor

   Paul Khavari

   Article

   Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

   Insert

   sgRNA backbone (F+E)
   

   Use

   Lentiviral

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PH-Btk-GFP

   Plasmid

   #51463

   Purpose

   Is a biosensor for PIP3

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   BTK (aa 1–177) (BTK Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TagRFP-T-EEA1

   Plasmid

   #42635

   Depositor

   Silvia Corvera

   Article

   Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

   Insert

   EEA1 (EEA1 Human)
   

   Tags

   TagRFP-T

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)- e18

   Plasmid

   #131670

   Purpose

   Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. RET-V5

    Plasmid

    #139627

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    RET (RET Human)
    

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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