We narrowed to 10,873 results for: Kars;

1. pLIC-SM-Myc

   Plasmid

   #111189

   Purpose

   High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster Myc Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Use

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

   Plasmid

   #168806

   Purpose

   PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   ASCL1-P2A-DLX2-T2A-mCherry
   

   Use

   Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-mH6-Cas12c1

   Plasmid

   #120872

   Purpose

   Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL17_Cas9-His Δcys M1C

   Plasmid

   #206251

   Purpose

   Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-mCherry-LKB1(WT)

   Plasmid

   #84639

   Purpose

   mCherry-tagged, wild-type LKB1

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   mCherry-LBK1 (STK11 Synthetic, Human)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC025

   Plasmid

   #227597

   Purpose

   Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-mH6-Cas12c2

   Plasmid

   #120873

   Purpose

   Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c2
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKERep

   Plasmid

   #211826

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PU.1-IRES-mCherry

    Plasmid

    #80140

    Purpose

    retroviral expression of PU.1 and mCherry

    Depositor

    Ellen Rothenberg

    Article

    Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

    Insert

    PU.1 (Spi1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-mH6-Cas12g1

    Plasmid

    #120879

    Purpose

    Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12g1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Psicheck2 PTPRN2 full length 3'UTR

    Plasmid

    #26994

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    PTPRN2 Full length 3' UTR (PTPRN2 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

14. ClCry4 PHR

    Plasmid

    #160552

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 HR
    

    Use

    Tags

    Hexa his

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS415_pGal-nCas9(D10A)

    Plasmid

    #79618

    Purpose

    Expresses nCas9(D10A) in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC026

    Plasmid

    #227598

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C2 RanGAP

    Plasmid

    #13378

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTH761-CEN-mCherry_v4

    Plasmid

    #40599

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mCherry ORF partially codon optimised for yeast e…

    Promoter

    GPD (TDH3)

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMax-E2F1

    Plasmid

    #16007

    Depositor

    Olimpia Meucci

    Article

    Shimizu et al Neurobiol Dis. 2007 Jan . 25(1):17-26.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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