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Lasso 1

Plasmid

#69935

Purpose

The first of the two RNA lassos required for the RNA chain construction

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 1

Use

Synthetic Biology

Promoter

TAATACGACTCACTATAGGG

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Lasso 2

Plasmid

#69936

Purpose

The second of the two RNA lassos required for the RNA chain construction.

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 2

Use

Synthetic Biology

Promoter

TAATACGACTCACTATAGGG

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA3mt

Tags

Myc

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Article

Rieubon et al protocols.io 2025

Insert

nucA

Tags

His and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized ecoli residues - 22-266

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA4mt

Plasmid

#140463

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA4mt

Tags

Myc

Expression

Mammalian

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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GW1-pHRed

Plasmid

#31473

Purpose

genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Expression

Mammalian

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_D17

Plasmid

#84611

Purpose

Introduces DSB at D17 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_MFE1

Plasmid

#84612

Purpose

Introduces DSB at MFE1 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Article

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_XPR2

Plasmid

#84609

Purpose

Introduces DSB at XPR2 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_AXP

Plasmid

#84608

Purpose

Introduces DSB at AXP locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_A08

Plasmid

#84610

Purpose

Introduces DSB at A08 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAV0133

Plasmid

#133467

Purpose

Integration into fission yeast genome at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0356

Plasmid

#133468

Purpose

Integration into fission yeast genome at ade6 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0413

Plasmid

#133470

Purpose

Integration into fission yeast genome at his5 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0357

Plasmid

#133469

Purpose

Integration into fission yeast genome at lys3 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pOPH6

Plasmid

#40315

Depositor

Shaun Lott

Article

Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

Insert

Peptide:N-glycosidase F

Tags

6xHis and OmpA3

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pTK511

Plasmid

#114686

Purpose

Express Mem-BFP-iLID

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

Mem-BFP-iLID

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHCL150

Plasmid

#134456

Purpose

CmR, Para-popZ-rbs-msfGFPC-H3H4. Replicative plasmid for the expression of bait. C-terminal GFP fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

rbs-msfGFPC-H3H4

Promoter

Para

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pYCTK034

Plasmid

#176738

Purpose

The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).

Depositor

Victor Sourjik

Article

Krink et al bioRxiv 2021

Insert

bar1Sc

Use

Synthetic Biology

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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