We narrowed to 12,453 results for: BASE

1. pRLuc190UGA

   Plasmid

   #41047

   Depositor

   James Inglese

   Article

   Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   RLuc190UGA
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   in-frame UGA stop codon at codon 190 in the open …

   Promoter

   CMV

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pA-HYG OSCAR

   Plasmid

   #29640

   Depositor

   Scott Gold, David_L Andrews

   Article

   Paz et al Fungal Genet Biol. 2011 Mar 5. ():.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. ssFv_lenti_mutATMSPARK2

   Plasmid

   #195250

   Purpose

   express Thr to Ala mutant ATMSPARK2 in a lenti vector

   Depositor

   Xiaokun Shu

   Article

   Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

   Insert

   Thr to Ala mutant ATMSPARK2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ssfv

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCVpuro-mIFP

   Plasmid

   #96951

   Purpose

   Retroviral plasmid containing mIFP for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   Monomeric Infrared Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-miniTurbo-V5-CDK15_IDG-K

   Plasmid

   #158179

   Purpose

   Gateway destination clone of CDK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK15 (CDK15 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p184_LTJ_sgRNACD90.2

   Plasmid

   #82670

   Purpose

   sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting mouse CD90.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-D4

   Plasmid

   #37473

   Depositor

   Amy Palmer, Roger Tsien

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D4 second generation cameleon (calcium sensor)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ara

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

8. LMAPPW

   Plasmid

   #205657

   Purpose

   shRNAmir backbone for RNA interference, with Puromycin selection

   Depositor

   Dapeng Wang

   Article

   Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

   Insert

   miR-AB
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mCMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA221T7RNAP

   Plasmid

   #167974

   Purpose

   Expresses T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   T7RNAP (T7p07 Synthetic)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   TetR-Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRPaCas9

    Plasmid

    #111819

    Purpose

    Expresses Cas9 in T. brucei (2T1) cells

    Depositor

    David Horn

    Article

    Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. apt-ABE8e

    Plasmid

    #219937

    Purpose

    Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Com_93-aa_TadA8e
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJB158

    Plasmid

    #202601

    Purpose

    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHis17_ParM _2

    Plasmid

    #78202

    Purpose

    Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

    Depositor

    Martin Webb

    Article

    Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.

    Insert

    parM (K33A D63C T174A T175N D224C C287A)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    K33A D63C T174A T175N D224C C287A

    Promoter

    unknown

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHSP70B-CP6-FCU1

    Plasmid

    #165882

    Purpose

    Basic 5-fluorocytosine sensitivity cassette in Alpha2, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMU-AUTr

    Plasmid

    #61204

    Purpose

    Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LC3(ATG8)
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-TK-Firefly-Control

    Plasmid

    #122277

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Thymidine kinase

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p663-UBC-TurboID-V5-GSK3B_IDG-K

    Plasmid

    #158164

    Purpose

    Gateway destination clone of GSK3B (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-GSK3B (GSK3B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    TurboID-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET11b-SpyCatcher-HA2

    Plasmid

    #165991

    Purpose

    SpyCatcher-fused influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-HA2
    

    Use

    Tags

    Foldon, HisTag, and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSplit-CO-hDib1

    Plasmid

    #58788

    Purpose

    Express LucC-hDib1 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHSP70B-CP6-nptII

    Plasmid

    #165876

    Purpose

    Basic G418 resistance cassette in Alpha1, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge