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p3E-V5-mScarlet-I_SV40pA (JDW 968)

Plasmid

#224529

Purpose

A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-REPORT (PGK) 5xGRE-NanoLuc

Plasmid

#207170

Purpose

GAl4 response element driven NanoLuc Lentiviral reporter

Depositor

David Bersten

Article

Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

Inserts

NanoLuciferase
d2eGFP
5x GRE

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

3x SV40 NLS and PEST

Promoter

Gal4 response element minP and PGK

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/GD-IRES-GFP-CTNNB1

Plasmid

#192868

Purpose

Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

Depositor

David Largaespada

Article

Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

Inserts

luc+
EGFP
CTNNB1

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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R725-X09-635: His6-tev-Hs.SPRED1(13-125)

Plasmid

#159581

Purpose

Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)

Tags

His6-tev

Expression

Insect

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-HtrA1-PDZ-1

Plasmid

#250091

Purpose

Bacterial expression of HtrA1 PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

HtrA1 (1/1) (Htra1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-1-PDZ-4

Plasmid

#250101

Purpose

Bacterial expression of Magi-1 PDZ domain 4

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-1 (4/6) (Magi1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-1-PDZ-6

Plasmid

#250102

Purpose

Bacterial expression of Magi-1 PDZ domain 6

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-1 (6/6) (Magi1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Chapsyn-110-PDZ-2

Plasmid

#250074

Purpose

Bacterial expression of Chapsyn-110 PDZ domain 2

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Chapsyn-110 (2/3) (Dlg2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Chapsyn-110-PDZ-3

Plasmid

#250075

Purpose

Bacterial expression of Chapsyn-110 PDZ domain 3

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Chapsyn-110 (3/3) (Dlg2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Lin7c-PDZ-1

Plasmid

#250096

Purpose

Bacterial expression of Lin7c PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Lin7c (1/1) (Lin7c Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-GRASP55-PDZ-1

Plasmid

#250087

Purpose

Bacterial expression of GRASP55 PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

GRASP55 (1/1) (Gorasp2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-S2 (JDW 1529)

Plasmid

#242605

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-S2. H2B for nuclear labeling

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-S2

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-H (JDW 1530)

Plasmid

#242604

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-H. H2B for nuclear labeling

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-H

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-SV40-pA (JDW 1417)

Plasmid

#242570

Purpose

Gateway 3' entry clone containing mCherry followed by an SV40 polyA.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCherry stop SV40 pA

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mRuby2-3xMyc (JDW 1246)

Plasmid

#242554

Purpose

Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mRuby2

Use

Gateway Cloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Puro-DEST (JDW 925)

Plasmid

#229808

Purpose

A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pXMLC2-DEST (JDW 7)

Plasmid

#229833

Purpose

A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway Cloning

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-BFP (JDW 491)

Plasmid

#229834

Purpose

A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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