We narrowed to 5,085 results for: mcs

1. pTripZ-B2

   Plasmid

   #163924

   Purpose

   For making a cell line that expresses doxycyline-inducible RNAi suppressor protein B2 for increasing titer of lentivirus with bidirectional promoters during virus production.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   Flock house virus B2 protein (FHVs1gp2 )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC-Flag-SOCS1-Y80D

   Plasmid

   #129516

   Purpose

   Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 80 to Aspartic acid

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-Flag-SOCS1-Y80F

   Plasmid

   #129517

   Purpose

   Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 80 to Phenylalanine

   Promoter

   cmv

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Empty Bridge Control Zfp462-Klf4SE

   Plasmid

   #127666

   Purpose

   Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   gRNAs 129, 135, 115, 117
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFL-SV40-TAK1 5'-UTR

   Plasmid

   #115356

   Purpose

   firefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNA

   Depositor

   Ming-Chih Lai

   Article

   Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

   Insert

   TAK1 5'-UTR (MAP3K7 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H

   Plasmid

   #108374

   Purpose

   Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with partial TNFalpha control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, TNF Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HDAC4*

   Plasmid

   #71402

   Purpose

   CAG promoter, mammalian expression

   Depositor

   Bo Chen

   Article

   Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

   Insert

   HDAC4* (HDAC4 Human)
   

   Expression

   Mammalian

   Mutation

   After aa126, insertion leads to reading frame shi…

   Promoter

   CAG

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. KHBD00012

   Plasmid

   #34174

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG8328 (HLHmdelta Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. C321.∆A ompT- rne- endA- lon-

   Bacterial Strain

   #182775

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Ampicillin

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

10. C321.∆A T7RNAP ompT- rne- lon-

    Bacterial Strain

    #182778

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

11. C321.∆A T7RNAP ompT- rne-

    Bacterial Strain

    #182777

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

12. C321.∆A ompT- rne- endA-

    Bacterial Strain

    #182774

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Ampicillin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

13. JEC027

    Bacterial Strain

    #180310

    Purpose

    E. coli MG1655 strain with knockout of endogenous Type I-E CRISPR Cas system

    Depositor

    James Chappell

    Article

    Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

    Bacterial Resistance

    None

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

14. rEc.13.delA

    Bacterial Strain

    #69495

    Purpose

    Recoded strain from MG1655, prfA deleted

    Depositor

    Michael Jewett

    Article

    Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

    Bacterial Resistance

    Ampicillin

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

15. C321.∆A ompT- rne- lon-

    Bacterial Strain

    #182773

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Ampicillin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

16. pCDH-CMV-mCherry-EF1 Hygro

    Plasmid

    #129440

    Purpose

    express mCherry

    Depositor

    Oskar Laur

    Insert

    mcherry
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-UBC-LUC-EF1 Hygro

    Plasmid

    #129436

    Purpose

    express LUC

    Depositor

    Oskar Laur

    Insert

    LUC
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1s

    Plasmid

    #72484

    Purpose

    Express gene of interest under truncated EF-1 promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCDH-CMV-Nluc

    Plasmid

    #73038

    Purpose

    Express Nluc reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTol2-Synapse reporters

    Plasmid

    #196899

    Purpose

    zebrafish expression

    Depositor

    Reinhard Köster

    Insert

    Synapse reporters
    

    Use

    Zebrafish expression

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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