We narrowed to 15,577 results for: GRN;

1. PX458-eBFP2-sgRNA_CTCF_ZF1

   Plasmid

   #233090

   Purpose

   Expression vector for a sgRNA against the mouse CTCF ZF1 region and SpCas9-T2A-eBFP2.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   spCas9-T2A-eBFP2 (Ctcf )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. NOLC1 atgRNA pDY2250

   Plasmid

   #219860

   Purpose

   attachment site pegRNA for human NOLC1

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pABE-dGFP-gRNA

   Plasmid

   #226864

   Purpose

   Human gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEs

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCBE-dGFP-gRNA

   Plasmid

   #226862

   Purpose

   Human gRNA expression vector targeting the Y93H mutation of non-fluorescent EGFP in plasmid pCBE-dGFP; for use with CBEs

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA giantin/GOLGB1

   Plasmid

   #222315

   Purpose

   CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

   Insert

   gRNA targeting GOLB1 (GOLGB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. gRNA-10Xcapture-PuroR

   Plasmid

   #211496

   Purpose

   Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

   Depositor

   Antonella Fidanza, Lesley Forrester

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   U6-gRNA
   Ef1a-PuroR
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-nucGFP11-3xPax7gRNA

   Plasmid

   #224570

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHelper-CRISPRi-sgRNA

   Plasmid

   #221135

   Purpose

   Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with dCas9 handle
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119(SpeI)

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTBL3241 PB-AtoBpegRNA-mCherry

   Plasmid

   #226666

   Purpose

   PiggyBac cargo vector encoding A to B pegRNA marked with mCherry

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   AtoB pegRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL3401 BtoApegRNA-sfGFP

    Plasmid

    #226667

    Purpose

    PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    BtoA pegRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICH86966::AtU6p::sgRNA:NbVPE1a/b

    Plasmid

    #223218

    Purpose

    Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbVPE1a/b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6 promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH86966::AtU6p::sgRNA:NbCysP6

    Plasmid

    #223217

    Purpose

    Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbCysP6 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6 promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

    Plasmid

    #216170

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

    Plasmid

    #216171

    Purpose

    Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

    Plasmid

    #216172

    Purpose

    Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- Control- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

    Plasmid

    #216173

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPRv2 EGFP sgRNA4

    Plasmid

    #164934

    Purpose

    Expresses EGFP sgRNA4 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDR274-gsc2-sgRNA

    Plasmid

    #184818

    Purpose

    Used to synthesize gRNA targeting exon 2 of the gsc2 gene

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    gsc2-sgRNA
    

    Use

    CRISPR; Template for sgrna synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. hPLD3-sgRNA-Cas9_mcherry

    Plasmid

    #199342

    Purpose

    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. hPLD4-sgRNA-Cas9_mcherry

    Plasmid

    #199343

    Purpose

    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    sgRNA: accagtagtatgaagccacg

    Promoter

    human U6

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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