Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 8,688 results for: PRS

Showing: 2661 - 2680 of 8688 results

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Article

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

Plasmid

#221112

Purpose

FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3

Expression

Yeast

Promoter

STE3

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

Plasmid

#221114

Purpose

FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS416-CUP1p-TID-FRB-NLS-HA3

Plasmid

#228009

Purpose

Expresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

CUP1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CUP1p- TID-FKBP-NLS-HA3

Plasmid

#228011

Purpose

Expresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

CUP1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CHA1p-TID-FRB-NLS-HA3

Plasmid

#228013

Purpose

Expresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

CHA1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CHA1p- TID-FKBP-NLS-HA3

Plasmid

#228015

Purpose

Expresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

CHA1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BirA-FRB-NLS-HA3

Plasmid

#227985

Purpose

Expresses BirA-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BirA-FKBP-NLS-HA3

Plasmid

#227987

Purpose

Expresses BirA-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BioID2-FRB-NLS-HA3

Plasmid

#227989

Purpose

Expresses BioID2-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p- BioID2-FKBP-NLS-HA3

Plasmid

#227991

Purpose

Expresses BioID2-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p- BASU-FRB-NLS-HA3

Plasmid

#227993

Purpose

Expresses BASU-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p- BASU-FKBP-NLS-HA3

Plasmid

#227995

Purpose

Expresses BASU-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-TID-FRB-NLS-HA3

Plasmid

#227997

Purpose

Expresses TurboID-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-TID-FKBP-NLS-HA3

Plasmid

#227999

Purpose

Expresses TurboID-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-mTID-FRB-NLS-HA3

Plasmid

#228001

Purpose

Expresses miniTurboID-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-mTID-FKBP-NLS-HA3

Plasmid

#228003

Purpose

Expresses miniTurboID-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-HIS3p-TID-FRB-NLS-HA3

Plasmid

#228005

Purpose

Expresses TurboID-FRB-NLS fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

HIS3

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-HIS3p- TID-FKBP-NLS-HA3

Plasmid

#228007

Purpose

Expresses TurboID-FKBP-NLS fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

HIS3

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-MET25p-TID-FRB-NLS-HA3

Plasmid

#228017

Purpose

Expresses TurboID-FRB-NLS fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 2661 - 2680 of 8688 results

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