We narrowed to 8,172 results for: hwa

1. pLJM1-FLAG-metap2

   Plasmid

   #100510

   Purpose

   Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   METAP2 (METAP2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GEF-H1 FLARE212.pa

   Plasmid

   #134930

   Purpose

   GEF-H1 biosensor that does not have GEF activity.

   Depositor

   Klaus Hahn

   Article

   Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

   Insert

   GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
   

   Use

   Tags

   None.

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. hCMV-IE1:GrRenilla

   Plasmid

   #118067

   Purpose

   Transcriptional unit for constitutive expression of GrRenilla in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:GreenRenilla:bGHF
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GEF-H1 FLARE191.pa

   Plasmid

   #134931

   Purpose

   GEF-H1 biosensor that has GEF activity.

   Depositor

   Klaus Hahn

   Article

   Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

   Insert

   GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
   

   Use

   Tags

   None.

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hCMV-IE1:FLuc

   Plasmid

   #118063

   Purpose

   Transcriptional unit for constitutive expression of FLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:FLuc:bGHF
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-pr-Kaede (Kaede-A69T)

   Plasmid

   #99228

   Purpose

   Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-Kaede
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A69T for primed conversion applications

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pF420

   Plasmid

   #123659

   Purpose

   Produces cofactor F420 in E. coli

   Depositor

   Colin Jackson

   Article

   Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

   Inserts

   CofD
   FbiB
   FbiD
   FbiC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_R0040

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG mEmerald-LAMP1

   Plasmid

   #168513

   Purpose

   Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

   Depositor

   Franck Polleux

   Article

   Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFNC-3

   Plasmid

   #233296

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Inserts

   BxbI phage integrase
   sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PEM7 and unknown

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101297

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-906 of alpha-Catenin

    Promoter

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5xNF-kb_RE

    Plasmid

    #118050

    Purpose

    GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    5 copies of the NF-kb DNA binding motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH3R-mCherry-N1

    Plasmid

    #84327

    Purpose

    Histamine-3 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H3R-mCherry (HRH3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-NPC1(P691S)-FLAG

    Plasmid

    #164973

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P691S

    Promoter

    EF1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CABE-T3.1

    Plasmid

    #193267

    Purpose

    Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T3.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-NPC1(F503/4A)-FLAG

    Plasmid

    #164974

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F503A and F504A

    Promoter

    EF1a

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherry-CIBN-CreC

    Plasmid

    #26889

    Purpose

    Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIBN-CreC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1-170 of CIB1, aa 106-343 of Cre

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. Renilla luciferase-Pol III

    Plasmid

    #37380

    Depositor

    Norbert Perrimon

    Article

    Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

    Insert

    PolIII-Renilla control reporter (RpIII128 Fly)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA PolIII 128 subunit (RpIII128)

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pRSFDuet-1_mNeongreen-Etanercept

    Plasmid

    #182420

    Purpose

    Bacterial expression of mNeongreen-Etanercept fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-Etanercept (TNFRSF1B Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV5 MEKK3 WT

    Plasmid

    #12186

    Depositor

    Gary Johnson

    Article

    Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

    Insert

    MEKK3 (Map3k3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pCDNA3-Flag MKK6(K82A)

    Plasmid

    #13519

    Depositor

    Roger Davis

    Article

    Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

    Insert

    MKK6(K82A) (MAP2K6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Lys-82Ala

    Promoter

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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