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We narrowed to 7,278 results for: cas9 plasmid

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Showing: 2681 - 2700 of 7278 results

  1. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)

    Viral Prep
    #112677-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  2. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

    Viral Prep
    #112677-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only

  3. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)

    Viral Prep
    #112677-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  4. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

    Viral Prep
    #112677-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  5. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)

    Viral Prep
    #112677-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  6. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)

    Viral Prep
    #112677-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only

  7. pCMV-ABE-P48R

    Plasmid
    #161816
    Purpose
    Expresses ABE-P48R in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    161816-plasmid-map-sequence-id-320046

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  8. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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  9. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

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  10. pMV_AA104

    Plasmid
    #245326
    Purpose
    Cas9 CRISPRa positive control guide; targets CD4
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD4 (CD4 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245326-plasmid-map-sequence-id-488034

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  11. pX335 Mouse 5' Srcap gRNA B

    Plasmid
    #127905
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127905-plasmid-map-sequence-id-249688

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  14. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

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  17. AAV-CMV-scFVtet2_bGHpA

    Plasmid
    #177352
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    CMV promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177352-plasmid-map-sequence-id-353673

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Showing: 2681 - 2700 of 7278 results