We narrowed to 6,890 results for: nls

1. PX458-3xHA-SpCas9

   Plasmid

   #130968

   Purpose

   3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   3xHA-NLS-SpCas9
   

   Use

   CRISPR

   Tags

   3x HA, EGFP, and NLS

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB–DualPE

   Plasmid

   #235161

   Purpose

   For plant prime editing including large DNA fragment editing in wheat plants or other monocotyledons

   Depositor

   Zong Yuan

   Article

   Zhao et al Nat Plants. 2025 Feb;11(2):191-205. doi:

   Inserts

   Csy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
   CmYLCV-epegRNA-CaMV poly(A) signal
   

   Use

   CRISPR

   Mutation

   Detailed in manuscript

   Available Since

   Oct. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. YFP-MEKK3

   Plasmid

   #12189

   Depositor

   Gary Johnson

   Article

   Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

   Insert

   MEKK3 (Map3k3 Mouse)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pMKO shRNA Bad

   Plasmid

   #17237

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bad shRNA (BAD Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pMKO shRNA Bmf

   Plasmid

   #17236

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bmf shRNA (BMF Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3 mouse Bmf

   Plasmid

   #17244

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bmf (Bmf Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

7. Pprm1-luciferase; his-3

   Plasmid

   #137728

   Purpose

   Communication-activated luciferase in Neurospora crassa

   Depositor

   Louise Glass

   Article

   Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

   Inserts

   Luciferase
   Pprm-1 Promoter
   

   Use

   Luciferase; Neurospora crassa expression

   Mutation

   Codon-optimized for N. crassa

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMH-HA

   Plasmid

   #101767

   Purpose

   Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH-MYC

    Plasmid

    #101765

    Purpose

    Gateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion protein

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJ915

    Plasmid

    #69090

    Purpose

    Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

    Depositor

    Jennifer Doudna

    Article

    Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

    Insert

    cas9
    

    Tags

    MBP, hexa His, and two SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-EGFP

    Plasmid

    #89871

    Purpose

    EGFP reporter vector for iTango system

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR1265

    Plasmid

    #111094

    Purpose

    lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p4455 FLAG-hPLIC-2

    Plasmid

    #8661

    Depositor

    Peter Howley

    Article

    Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

    Insert

    hPLIC-2 (UBQLN2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a-dCasRx-RBM25

    Plasmid

    #221001

    Purpose

    Transient expression of dCasRx-RBM25. EF1a promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. enOgeuIscB with ωRNA-v13

    Plasmid

    #220940

    Purpose

    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing

    Depositor

    Weixin Tang

    Article

    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

    Inserts

    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMH-MBP

    Plasmid

    #101768

    Purpose

    Gateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion protein

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PSD95-TS:YFP

    Plasmid

    #42225

    Depositor

    Roger Tsien

    Article

    Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

    Insert

    PSD95
    

    Tags

    TS:YFP

    Expression

    Mammalian

    Available Since

    Feb. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. p4458 FLAG-hPLIC-1

    Plasmid

    #8663

    Depositor

    Peter Howley

    Article

    Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

    Insert

    hPLIC-1 (UBQLN1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only