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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 2681 - 2700 of 18824 results

  1. pICSL50059

    Plasmid
    #245614
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag 2xBP NLS (2xGKRTADGSEFEPKKKRKV)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245614-plasmid-map-sequence-id-495524

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  6. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

    Plasmid
    #133802
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AcrIIA5
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV and T7
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133802-plasmid-map-sequence-id-267958

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  7. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

    Plasmid
    #133801
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AcrIIA4
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV and T7
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133801-plasmid-map-sequence-id-267964

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  8. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid
    #115137
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AcrVA2.1 anti-CRISPR protein
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115137-plasmid-map-sequence-id-220492

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  12. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

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  13. JAB3278

    Plasmid
    #190402
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Publication
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
    Expression
    Plant
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    190402-plasmid-map-sequence-id-393458

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  15. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid
    #239293
    Purpose
    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system
    Depositor
    Anita Hopper
    Publication
    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.
    Inserts
    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    Expression
    Yeast
    Mutation
    Contains M2-SE-G72P mutations, described in Addge…
    Promoter
    TDH3 and tetracycline-responsive element (TRE) an…
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    239293-plasmid-map-sequence-id-476828

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  17. pLJM1-CRS-Halo-NLS

    Plasmid
    #250859
    Purpose
    lentiviral vector for expressing HaloTag protein tagged with both a GDOWN1 cytoplasmic retention signal (CRS) and a SV40 NLS
    Depositor
    Britt Glaunsinger
    Insert
    HaloTag (POLR2M Human)
    Use
    Lentiviral
    Tags
    GDOWN1 (aa 250-368) and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Feb. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    250859-plasmid-map-sequence-id-498765

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Showing: 2681 - 2700 of 18824 results