Addgene

We narrowed to 8,369 results for: pcDNA3

Showing: 2681 - 2700 of 8369 results

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pCDNA3 T7 BimL(S58A,DE)

Plasmid

#25056

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58A,DE) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine changed leucine 92 …

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (AAA)

Plasmid

#24244

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AAA) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

changed Serine 44 to Alanine changed Threonine 5…

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (T56A)

Plasmid

#24247

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(T56A) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to alanine

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (S44A)

Plasmid

#24245

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed Serine 44 to Alanine

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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1236 pcDNA3 Myr HA Akt3

Plasmid

#9017

Depositor

William Sellers

Insert

Akt3 (AKT3 Human)

Use

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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1227 pcDNA3 Myr HA Akt2

Plasmid

#9016

Depositor

William Sellers

Insert

Akt2 (AKT2 Human)

Use

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Peredox-mCherry-NLS

Plasmid

#32384

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Use

Tags

Nuclear Localization Seq

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-spikeRBD::∆ACE2 SURF

Plasmid

#206957

Purpose

Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

Depositor

Xiaokun Shu

Article

Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

Inserts

sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
ACE2 (ACE2 Human)

Use

Tags

cSURF, mCherry, and nSURF

Expression

Mammalian

Mutation

17-615 and RBD (319-541)

Promoter

CMV

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-544

Plasmid

#53548

Purpose

mammalian expression of TPP1 full length

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
Nrf1-Myc pcDNA3.1(+)-C-Myc

Plasmid

#169125

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hNrf1 (NFE2L1 Human)

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC S62D

Plasmid

#203437

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Use

Tags

2 x HA

Expression

Mammalian

Mutation

S62D

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC S62A

Plasmid

#203436

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Use

Tags

2 x HA

Expression

Mammalian

Mutation

S62A

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-isoform a

Plasmid

#225447

Purpose

Express EGFP-fusion protein of FXR1 isoform a

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-CMV-bArrestin2-TEV

Plasmid

#107245

Purpose

Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ARRB2

Use

Tags

TEV(NIA), tobacco etch virus NIA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-10His-mStayGold (E138D)

Plasmid

#211363

Purpose

Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Use

Tags

10xHis-tag

Expression

Mammalian

Mutation

E138D

Promoter

CMV

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4 human alpha5 full

Plasmid

#221396

Purpose

Mammalian expression of human integrin alpha5 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 full length (ITGA5 Human)

Use

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Lari-BFP2-TSERex

Plasmid

#123311

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Lari-BFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pcDNA3

Plasmid

#18955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Hygro EYFP H148Q

Plasmid

#25873

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q

Use

Tags

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q mutation

Promoter

Available since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only