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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,702 results for: NSI;

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Showing: 2701 - 2720 of 12702 results

  1. p4m

    Plasmid
    #120936
    Purpose
    MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23151
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120936-plasmid-map-sequence-id-235945

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  2. EcMscL

    Plasmid
    #92418
    Purpose
    E. coli Mechanosensitive Channel of Large Conductance
    Depositor
    Douglas Rees
    Insert
    MscL (mscL E. coli)
    Tags
    6x Histidine Tag and Thrombin Cleavage Site betweā€¦
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92418-plasmid-map-sequence-id-183401

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  3. C83m

    Plasmid
    #120994
    Purpose
    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pir protein required for R6K ori
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120994-plasmid-map-sequence-id-236103

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  4. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

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  5. T16m

    Plasmid
    #121035
    Purpose
    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P55 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121035-plasmid-map-sequence-id-245903

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  6. T17m

    Plasmid
    #121036
    Purpose
    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121036-plasmid-map-sequence-id-245428

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  7. pBP-P_TALEsp1_PUPsp1-phlF_PPhlF

    Plasmid
    #204080
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    TALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204080-plasmid-map-sequence-id-413895

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  8. pBP-P_lacI-Ptac-lacO

    Plasmid
    #204072
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    lacI repressor and Ptac-lacO IPTG-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204072-plasmid-map-sequence-id-413888

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  9. U24m

    Plasmid
    #121026
    Purpose
    MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    RiboJ + BCD2
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121026-plasmid-map-sequence-id-238071

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  10. T14m

    Plasmid
    #121033
    Purpose
    MoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S1P13 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121033-plasmid-map-sequence-id-236356

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  11. T15m

    Plasmid
    #121034
    Purpose
    MoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S3P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121034-plasmid-map-sequence-id-245426

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  12. C90m

    Plasmid
    #120998
    Purpose
    MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    NahRsfGFP fusion
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120998-plasmid-map-sequence-id-236128

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  13. C92m

    Plasmid
    #121000
    Purpose
    MoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).
    Depositor
    Richard Murray
    Insert
    sfCFPpdt3 (Chen,Bennett)
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121000-plasmid-map-sequence-id-238058

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  14. C101m

    Plasmid
    #121005
    Purpose
    MoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).
    Depositor
    Richard Murray
    Insert
    sfCFP with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121005-plasmid-map-sequence-id-236158

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  15. C20m

    Plasmid
    #120961
    Purpose
    MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)
    Depositor
    Richard Murray
    Insert
    sfYFP-ssrA (has BbsI site)
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120961-plasmid-map-sequence-id-235979

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  16. C22m

    Plasmid
    #120963
    Purpose
    MoClo golden gate assembly CD part for mSpinach (fluorescent RNA aptamer for DFHBI, suitable for in vivo use; see DOI: 10.1021/sb400089c). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mSpinach
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120963-plasmid-map-sequence-id-236357

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  17. P37m

    Plasmid
    #120947
    Purpose
    MoClo golden gate assembly AB part for pRpa* (improved pRpa from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pRpa* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120947-plasmid-map-sequence-id-236378

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  18. P38m

    Plasmid
    #120948
    Purpose
    MoClo golden gate assembly AB part for pTra* (improved pTra from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pTra* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120948-plasmid-map-sequence-id-236376

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  19. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baciā€¦
    Expression
    Bacterial
    Promoter
    Pveg
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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Showing: 2701 - 2720 of 12702 results