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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 2721 - 2740 of 6562 results

  2. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Publication
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  3. pcDNA3-P4:gs:nls:CIBN

    Plasmid
    #135986
    Purpose
    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P4 coiled-coil fused to the CIBN domain
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135986-plasmid-map-sequence-id-269554

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  7. mCerulean-PCNA-19-SV40NLS-4

    Plasmid
    #55386
    Purpose
    Localization: Nucleus, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    3xSV40 NLS and mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  12. HBT_pcoCASphi_version1

    Plasmid
    #197931
    Purpose
    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal
    Depositor
    Steven Jacobsen
    Publication
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Insert
    pcoCasphi-2
    Use
    CRISPR
    Tags
    FLAG tag and SV40 NLS and nucleoplasmin NLS
    Expression
    Plant
    Promoter
    35SPPDK promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197931-plasmid-map-sequence-id-388007

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  13. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  16. pcDNA3-BG4-NLS-mCer

    Plasmid
    #211448
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Tags
    NLS-mCerulean
    Expression
    Mammalian
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211448-plasmid-map-sequence-id-419419

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  17. pcDNA3-BG4-NLS-mVen

    Plasmid
    #211449
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Tags
    NLS-mVenus
    Expression
    Mammalian
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211449-plasmid-map-sequence-id-419262

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  20. pPHR-iRFP713-NLS-VP16

    Plasmid
    #103823
    Purpose
    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
    Depositor
    Karsten Rippe
    Publication
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHR-iRFP713-VP16 (CRY2 Mustard Weed)
    Expression
    Mammalian
    Mutation
    VP16: G126T (silent, G42G)
    Promoter
    CMV
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    103823-plasmid-map-sequence-id-199372

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Showing: 2721 - 2740 of 6562 results