We narrowed to 4,795 results for: pcr
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Plasmid#230002PurposeExpresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-8E10-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-mCherry(LaM6) PAGER(TF)
Plasmid#229998PurposeExpresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaM6-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
GPR3-Tango
Plasmid#66350PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-no-pep
Plasmid#47439Purposecontrol FRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
Tags10 nm ER/K helix, HA, mCerulean, mCitrine, and no…ExpressionMammalianPromoterpCMVAvailable SinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
GPR113-Tango
Plasmid#66304PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mcherry Gamma 9 ---pcDNA3.1
Plasmid#64203PurposeG protein Gamma cone(9) subunit tagged with mcherry on the N terminusDepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mScarlet-gamma9
Plasmid#140097PurposeExpresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocationDepositorInsertGNGT2
TagsN-terminal mScarlet tagExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GPR32-Tango
Plasmid#66352PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-q-pep
Plasmid#47437PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
Tags10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…ExpressionMammalianPromoterpCMVAvailable SinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_GCGR-NTEV-TCS-GV-2xHA
Plasmid#194374PurposeSplit TEV assaysDepositorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
GPR25-Tango
Plasmid#66347PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
bArr1-ALFA
Plasmid#201496PurposeExpression of the beta-arrestin1 containing a C-terminal ALFA tag.DepositorAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_DRD1-NTEV-TCS-GV-2xHA
Plasmid#194358PurposeSplit TEV assaysDepositorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-RTP1S-Integration
Plasmid#129458PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses RTP1SDepositorInsertRTP1 (Rtp1 Mouse)
UseSynthetic BiologyExpressionMammalianMutationRemoval of the first 36 amino acids of the proteinPromoterTRE (Tet-On)Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N
Plasmid#113952Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, I132S and S212N su…PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 FS10
Plasmid#113956Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, S212N substitution…PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-tmeGFP-IRES-nls-mRuby2
Plasmid#63060PurposeExpresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertnls-mRuby2
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_AVPR2-NTEV-TCS-GV-2xHA
Plasmid#194370PurposeSplit TEV assaysDepositorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_S212N
Plasmid#113951Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, S212N substitutionPromoterCMVAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL23Cm6
Plasmid#109367PurposeHuman rod opsin chimera with intracellular loops 2-3 and C-terminus from mGluR6DepositorAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only