We narrowed to 10,877 results for: ALP

1. pCAG CAPN7 F98D-OSF

   Plasmid

   #193044

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   F98D

   Promoter

   Chicken Beta-actin

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-CAPN7-V18D-mCherry

   Plasmid

   #193046

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   V18D, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-CAPN7-C290S-mCherry

   Plasmid

   #193047

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C290S, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 CAPN7 V18D,F98D (1-165)

   Plasmid

   #193034

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   V18D, F98D, Residues 1-165

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSuper Retro GFP DGK alfa human

   Plasmid

   #224578

   Purpose

   To stably downregulate the expression of human DGK alfa in human cells

   Depositor

   Isabel Mérida

   Article

   Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

   Insert

   sh RNAi Diacylglycerol kinase alfa human (DGKA Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   H1

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. ADRA2B-DuET

   Plasmid

   #213178

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRA2B (ADRA2B Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-SopB(172-554)

   Plasmid

   #183664

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 172-554

   Promoter

   CMV

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-SopB(211-554)

   Plasmid

   #183665

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 211-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

   Plasmid

   #60394

   Purpose

   Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Budding Yeast, Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4), amino a…

   Promoter

   GAL

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

10. HNF4A_P2-198

    Plasmid

    #31065

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -198 to -1 of P2 HNF4A promoter

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. FR_HNF1A

    Plasmid

    #31104

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    HNF1A (HNF1A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. NRXN-bio-His

    Plasmid

    #51810

    Purpose

    Expresses full-length Neurexin-1-alpha precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    NRXN (NRXN1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-7 asyn A53T

    Plasmid

    #105727

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A53T (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A53T

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid

    #101299

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 and 698-906 of alpha-C…

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-AAV-sh[SNCA]

    Plasmid

    #75437

    Purpose

    knockdown alpha-synuclein in rat neurons

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    snca (Snca Rat)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101297

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-906 of alpha-Catenin

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-7 asyn A30P

    Plasmid

    #105726

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A30P (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A30P

    Promoter

    T7

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-PGC1(1-400)

    Plasmid

    #1029

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains only aa1-400

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

19. KG#205

    Plasmid

    #110881

    Purpose

    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

    Inserts

    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Asp 448 to Asn

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLNCX1 HA Akt1

    Plasmid

    #15990

    Depositor

    Morris Birnbaum

    Article

    Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only