We narrowed to 9,466 results for: CAG

1. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

   Plasmid

   #199457

   Purpose

   Expression of dCas13-VPR in mammalian cells

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   dCas13
   

   Use

   Lentiviral

   Tags

   VPR-T2A-HaloTag

   Expression

   Mammalian

   Mutation

   Nuclease-dead H133A and H1058A

   Promoter

   CAG

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

   Plasmid

   #52419

   Purpose

   Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   ERAS (ERAS Human)
   

   Promoter

   CAGGS

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

   Plasmid

   #168106

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   mCherry-KASH
   

   Use

   CRISPR and Cre/Lox

   Promoter

   CAG

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

   Plasmid

   #180156

   Purpose

   tdTomato cre reporter with Hipp11 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

   Plasmid

   #216321

   Purpose

   Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Luciferase + splice acceptor site
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216249

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgRANGAP.C1 (RANGAP1 Human)
   

   Use

   CRISPR; Endogenous tagging

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

   Plasmid

   #180153

   Purpose

   td-sfGFP dre reporter with Rosa26 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Mouse Targeting; Dre/rox

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

   Plasmid

   #104122

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   spine-jRGECO1a-P2A-QuasAr-dark Citrine
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

   Plasmid

   #196890

   Purpose

   Neuron-specific expression of human (h)Centrin fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mNeonGreen-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid

    #172479

    Purpose

    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid

    #114094

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (S542R/K607R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    S542R and K607R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

    Plasmid

    #171965

    Purpose

    Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a ITAM CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid

    #206115

    Purpose

    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (Cacna1a Rat)
    

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV/B-actin

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

    Plasmid

    #104566

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-p65
    

    Tags

    3x HA and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

    Plasmid

    #114726

    Purpose

    dCas9-VP160 expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    dCas9-VP160
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA

    Plasmid

    #196896

    Purpose

    Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    N-Cadherin-2xHA (Cdh1 Mouse)
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid

    #180151

    Purpose

    td-sfGFP cre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP

    Plasmid

    #196875

    Purpose

    Neuron-specific expression of LynGFP reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynEGFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Mutation

    D10A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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