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pEGFP-C1-hRabin8 L196A

Plasmid

#26727

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

L196A: GEF domain mutant.

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-hRabin8 F201A

Plasmid

#26728

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

F201A: GEF domain mutant

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Sport6 Baf60b

Plasmid

#24723

Depositor

Benoit Bruneau

Publication

Lickert et al Nature. 2004 Nov 4. 432(7013):107-12.

Insert

Baf60b (Smarcd2 Mouse)

Expression

Bacterial and Mammalian

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pCAT204

Plasmid

#134881

Purpose

Bacterial expression

Depositor

Thomas Howard

Publication

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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p285 TIR1_HA_mH11

Plasmid

#164855

Purpose

constitutive TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Publication

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A

Plasmid

#86921

Purpose

To express Ubl4A tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)-L-GFP

Plasmid

#193716

Purpose

Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

Depositor

Lukas Kapitein

Publication

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-560);

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET-mGinger1

Plasmid

#119784

Purpose

Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Publication

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mGinger1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pTL032

Plasmid

#80928

Purpose

14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxP

Depositor

Daniel Larson

Publication

Lenstra et al Mol Cell. 2015 Nov 19;60(4):597-610. doi

Insert

14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP

Expression

Bacterial

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pKM19

Plasmid

#123655

Purpose

Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

Depositor

Kevin Maringer

Publication

Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

Insert

Firefly luciferase

Use

Luciferase

Expression

Insect

Promoter

pIEx

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon delta182

Plasmid

#28041

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rubicon (RUBCN Human)

Tags

3xFlag and EGFP

Expression

Mammalian

Mutation

delta 1-182aa

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pKO2.1

Plasmid

#22674

Depositor

Robert Benezra

Publication

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

DTA

Use

Mouse Targeting

Mutation

pDTA for targeting vector generation. NotI linea…

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Publication

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFlagCMV2-UbcM8C86A

Plasmid

#12441

Depositor

Dong-Er Zhang

Publication

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UbcM8 (Ube2l6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

C86A

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
Self-targeting epegRNA library, +5 G>H (lDS004)

Pooled library

#227707

Purpose

CRISPR guide RNA library for prime editing

Depositor

Brittany Adamson

Publication

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Expression

Mammalian

Use

Lentiviral

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only
TRE-MPRA_minTK Library

Pooled library

#201014

Purpose

Massively parallel reporter assay (MPRA) library with minTK promoter

Depositor

Justin English

Publication

Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi

Expression

Mammalian

Use

Luciferase

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only
pAS_4xMma PylT_FLAG-PcKRS

Plasmid

#140022

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

PcKRS (pylS Methanosarcina bakeri)

Tags

FLAG

Expression

Mammalian

Mutation

M241F, A267S, Y271C, L274M

Promoter

EF1

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-mAvicFP1

Plasmid

#129509

Purpose

Expresses mAvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

mAvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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BL21ΔAC

Bacterial strain

#102261

Purpose

ompA, ompC double knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Publication

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial resistance

None

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only