We narrowed to 7,139 results for: SPL

1. pU1/Sm/3' Box_(GLuc)_TOP1

   Plasmid

   #68437

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U1

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET:GACT:GFP

   Plasmid

   #60734

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEP108E

   Plasmid

   #97394

   Purpose

   binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   sfGFP1-10OPT
   

   Use

   Tags

   Plasma membrane target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-N1IC

   Plasmid

   #47612

   Purpose

   bacterial expression of GST-myc-Notch1 IC

   Depositor

   Urban Lendahl

   Article

   Beatus et al Development. 1999 Sep;126(17):3925-35.

   Insert

   Notch1 intracellular domain (Notch1 Mouse)
   

   Use

   Tags

   GST and myc

   Expression

   Bacterial

   Mutation

   contains amino acids 1753-2531

   Promoter

   tac

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3-ySet2

   Plasmid

   #51698

   Purpose

   Expresses full length yeast Set 2 gene in a mamalian expression vector

   Depositor

   Tom Misteli

   Article

   Luco et al Science. 2010 Feb 19;327(5968):996-1000.

   Insert

   yeast Set2 (SET2 Budding Yeast)
   

   Use

   Tags

   C-terminal FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. PEP113E

   Plasmid

   #97399

   Purpose

   binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Peroxisome target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEBB-II-I-GFP alpha isoform

   Plasmid

   #22146

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

8. SNV-gfp-IRES-MCS

   Plasmid

   #53122

   Purpose

   Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

   Depositor

   Jeanette Hyer

   Article

   Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

   Type

   Empty backbone

   Use

   Retroviral; For expression in chicken cells

   Tags

   EGFP-IRES

   Expression

   Mutation

   Promoter

   SNV (spleen necrosis virus)

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBG-TFII-I gamma

   Plasmid

   #22156

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pB-bsd-Flag-Trl1

    Plasmid

    #126609

    Purpose

    Expresses N-terminal Flag-tagged yeast Trl1

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    tRNA ligase 1 (TRL1 Budding Yeast)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEBB-II-I-GFP gamma isoform

    Plasmid

    #22148

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pCMV/3' Box_(GLuc)_TOP2

    Plasmid

    #68435

    Purpose

    Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP2 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSiMPlh_N + pSiMPlh_C

    Plasmid

    #134316

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P254Q in hygR_gp41-1 N-intein

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. RFP-FKBP-U2AF35

    Plasmid

    #187969

    Purpose

    expresses U2AF35 with N-terminal tags RFP and FKBP

    Depositor

    Markus Wahl

    Article

    Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

    Insert

    U2AF35 (U2af1 Mouse)
    

    Use

    Cmv promoter

    Tags

    mRFP-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RFP-FKBP-PRPF38A

    Plasmid

    #187968

    Purpose

    expresses PRPF38A with N-terminal tags RFP and FKBP

    Depositor

    Markus Wahl

    Article

    Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

    Insert

    Prp38A (PRPF38A Synthetic, Human)
    

    Use

    Cmv promoter

    Tags

    mRFP-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6_(Gluc)_INT(GenPurpClon)

    Plasmid

    #68433

    Purpose

    General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct
    

    Use

    CRISPR; Cloning vector for generating int-like co…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6_(GLuc)_INT[Spinach2]

    Plasmid

    #68430

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Spinach2" aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cPE

    Plasmid

    #183549

    Purpose

    Expresses compact Prime Editor in mammalian cells

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    SpCas9 nickase (H840A), Reverse Transcriptase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBB-II-I-GFP beta isoform

    Plasmid

    #22147

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac-His-Flag-Timeless

    Plasmid

    #22892

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only