We narrowed to 709 results for: pcdna3 1 plasmid

1. pcDNA3-Flag-Tet1 CD Mut

   Plasmid

   #72218

   Purpose

   Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET1 catalytic domain (cata…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+)-RasAR(R89L)-NES

   Plasmid

   #205568

   Purpose

   RasAR negative-control mutant plasmid. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR(R89L)-NES (RAF1 Synthetic, Human)
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Arg 89 mutated to Leu in Raf1 RBD region.

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-MYO10-HMM-Nanotrap

   Plasmid

   #87255

   Purpose

   Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Use

   Tags

   GFP-nanotrap

   Expression

   Mammalian

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   CMV

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/TO-myc-BirA*-cCE

   Plasmid

   #112713

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   BirA* (BirA R118G), HIV Rev nuclear localization …

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

   Plasmid

   #182685

   Purpose

   Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and NA

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

   Plasmid

   #238007

   Purpose

   300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

   Depositor

   Agnel Sfeir, Simon Powell

   Article

   Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

   Insert

   AAVS1 (AAVS1, PPP1R12C Human)
   

   Use

   Rna donor to study rt-dsbr

   Tags

   Expression

   Mammalian

   Mutation

   3bp insertion signature (ATC) at Cas9 cut site in…

   Promoter

   CMV

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

   Plasmid

   #182686

   Purpose

   Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   NA

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. (383) pcDNA3.1-HA-nBC2-OGA(544-706)

   Plasmid

   #168100

   Purpose

   HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nBC2-OGA(544-706)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   OGA truncated to contain residues 544-706

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

   Plasmid

   #60606

   Purpose

   Mammalian Display vector

   Depositor

   Wayne Marasco

   Article

   Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

   Insert

   PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

    Plasmid

    #168097

    Purpose

    HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nGFP-(EAAAK)4-OGA(544-706)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid

    #168099

    Purpose

    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nUbc-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

    Plasmid

    #168102

    Purpose

    HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nEPEA-(EAAAK)3-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BcLOV4 fusion tools screening plasmid #1

    Plasmid

    #174511

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. acat 1-906

    Plasmid

    #24194

    Depositor

    David Rimm

    Insert

    alpha catenin (CTNNA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full length

    Promoter

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

    Plasmid

    #21845

    Depositor

    David Ron

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BcLOV4 fusion tools screening plasmid #2

    Plasmid

    #174512

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    

    Use

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Neurexin-1-Beta scFv [N170A/1]

    Plasmid

    #190534

    Purpose

    Mammalian Expression of Neurexin-1-Beta scFV. Derived from hybridoma N170A/1.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Neurexin-1-Beta (Homo sapiens) recombinant scFV (NRXN1 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NOVA1 eGFP

    Plasmid

    #61275

    Purpose

    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    NOVA1 (NOVA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IP3 receptor, type 1 scFv [L24/1]

    Plasmid

    #206755

    Purpose

    Mammalian Expression of IP3 receptor, type 1 scFV. Derived from hybridoma L24/1 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    IP3 receptor, type 1 (Rattus norvegicus) recombinant scFV (Itpr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCD/NL-BH∆1

    Plasmid

    #41791

    Purpose

    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    HIV-1 Gag/Pol and RRE sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge