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pmCellFree_KA1 ZIKV E

Plasmid

#145341

Purpose

Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Envelope

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p2.0VPI.EGFP

Plasmid

#40868

Depositor

Harold Gainer

Article

Ponzio et al PLoS One. 2012;7(11):e48860. doi: 10.137

Insert

mouse vasopressin promoter

Use

AAV

Expression

Bacterial and Insect

Promoter

mouse vasopressin

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-CaM

Plasmid

#193816

Purpose

CMV overexpression of ChemoG-CaM in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-CaM

Expression

Mammalian

Mutation

EGFP:E116D, A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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TAp63gamma-(3X)HA

Plasmid

#26977

Depositor

David Sidransky

Article

Upadhyay et al Biochem Biophys Res Commun. 2007 May 18.

Insert

TAp63gamma (TP63 Human)

Tags

3XHA

Expression

Mammalian

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS5

Plasmid

#111760

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS5-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1c

Plasmid

#134832

Purpose

Expression of rat Myo 1c (Myr 2) in mammalian cells. N-terminal GFP-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Sci. 1995 Dec;108 ( Pt 12):3775-8

Insert

Myo 1c (rat) with 3' UTR (Myo1c Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD2

Plasmid

#102976

Purpose

Suppression of SOD2

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD2 (SOD2 Human)

Use

Lentiviral and RNAi

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCLR2070

Plasmid

#28063

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Contains N496D point mutation in FokI domain, as …

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCLR2068

Plasmid

#28062

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Contains H537R point mutation in FokI domain, as …

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Positron-ST

Plasmid

#129267

Purpose

Cre-dependant expression of soma-localized Positron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

AAV

Expression

Mammalian

Mutation

D92N, E199V

Promoter

hsyn

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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SiC-V1

Plasmid

#133041

Purpose

SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pWZLblasti-CTR1

Plasmid

#53157

Purpose

Expresses human CTR1 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
LNCX TbetaRII DN

Plasmid

#12640

Depositor

Rik Derynck

Article

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

TGF-beta receptor II (TGFBR2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: truncated cytoplasmically

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR V2 sgIREB2_2

Plasmid

#169886

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-TWIST2

Plasmid

#197935

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

Depositor

Kevin Janes

Article

Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

Insert

TWIST2 (TWIST2 Human)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCX-2A-2A

Plasmid

#74670

Purpose

based on pCX-C1 with F2A1/F2A2 inserted

Depositor

Roberto Giovannoni

Article

De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

Type

Empty backbone

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Gal4DBD-GBP2

Plasmid

#49439

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP2-Gal4 DNA binding domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG5

Plasmid

#193807

Purpose

CMV overexpression of ChemoG5 in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG5

Tags

10xHis

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP1-10gly-lexADBD

Plasmid

#50795

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP1-lexA DNA binding domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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