We narrowed to 4,190 results for: Pet2

1. USP7 delta C-terminus

   Plasmid

   #234074

   Purpose

   Expresses USP7 mutant in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 1-1080 (USP7 Human)
   

   Use

   Tags

   6xHis cleavable with thrombin

   Expression

   Bacterial

   Mutation

   E547 mutated to stop codon

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAIP4_5HFD

   Plasmid

   #234189

   Purpose

   Heterologous protein expression of disks large homolog 4 (PSD-95) (SAP90) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   5HFD (Dlg4 Rat)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAIP4_4QMD

   Plasmid

   #234174

   Purpose

   Heterologous protein expression of envoplakin in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4QMD (EVPL Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAM299

   Plasmid

   #224258

   Purpose

   MCMUbHis

   Depositor

   Andreas Martin

   Article

   Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

   Insert

   MC-Ub-3C-His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TDRD3:JMC006-H10:C42283

   Plasmid

   #183981

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   TDRD3 in Complex with anti-TDRD3 FAB (TDRD3 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pXalb_IF1_Cas2-3

   Plasmid

   #178769

   Purpose

   Expression of X. albilineans type I-F1 cas2-3

   Depositor

   Chase Beisel

   Article

   Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

   Insert

   X. albilineans type I-F1 cas2-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5JJW (USP15_SART3)

   Plasmid

   #101880

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP15_SART3 (USP15, SART3 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. MSP824

   Plasmid

   #98803

   Purpose

   Expression Vector

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   pET28 MBP-ENLYGQS-TEV V209M, W211I, M218F
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V209M, W211I, M218F

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK605

   Plasmid

   #72530

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK623

    Plasmid

    #72540

    Purpose

    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK289

    Plasmid

    #71553

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-39 to alanine (please see deposito…

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK501

    Plasmid

    #72246

    Purpose

    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    T. J. Kappock

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gln206 to Arg and changes tryptophan-151 to pheny…

    Promoter

    T7 and endogenous

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK502

    Plasmid

    #71582

    Purpose

    Produces Acetobacter aceti 1023 putative SixA with C-terminal His6 tag (SixAH6)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

    Insert

    phosphohistidine phosphatase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK304

    Plasmid

    #72225

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes alanine-368 to aspartate

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK303

    Plasmid

    #72224

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes alanine-367 to aspartate

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK579

    Plasmid

    #72426

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites

    Depositor

    T. J. Kappock

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    4 silent mutations introduce unique internal SpeI…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK522

    Plasmid

    #72510

    Purpose

    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK600

    Plasmid

    #71605

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, histidine…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2GSF

    Plasmid

    #51363

    Depositor

    Cheryl Arrowsmith

    Insert

    2GSF (EPHA3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. GIMAP1 (3LXW)

    Plasmid

    #25336

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GIMAP1:E25-R253 (GIMAP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids E25-R253

    Promoter

    Available since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits