We narrowed to 6,486 results for: Pet2

1. His6-Twin-Strep-SUMO-pET28a-EfaCas2

   Plasmid

   #112794

   Purpose

   To express enterococcus faecalis Cas2 protein in E. coli

   Depositor

   Ailong Ke

   Article

   Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

   Insert

   EfaCas2
   

   Use

   Tags

   His6-Twin-Strep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FabF (KASII) pet28 N-terminal His Tag

   Plasmid

   #75017

   Purpose

   Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

   Depositor

   Michael Burkart

   Article

   Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

   Insert

   FabF (fabF E. coli)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pET28b – E. coli RecE residues 564-868

   Plasmid

   #104534

   Purpose

   Expresses E. coli RecE residues 564-868

   Depositor

   Charles Bell

   Article

   Zhang et al Structure. 2009 May 13;17(5):690-702. do

   Insert

   RecE residues 564-866 (C-terminal nuclease domain)
   

   Use

   Tags

   6X-His tag followed by thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21 Catalytic Domain of MALT1 Catalytic Mutant

   Plasmid

   #48971

   Purpose

   Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 (MALT1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains AA 329-566; Catalytic Mutant C464A

   Promoter

   T7

   Available since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

5. Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

   Plasmid

   #111052

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AB (325-557, Δ 368-523)

   Promoter

   T7

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-10XHis-GST-HRV-Her1-dL5

   Plasmid

   #73216

   Purpose

   Bacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

   Insert

   10XHis-GST-HRV-Her1-dL5 (EGFR Synthetic, Human, Schistosoma japonicum)
   

   Use

   Tags

   10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …

   Expression

   Bacterial

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   T7

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-10XHis-GST-HRV-dL5-Her1

   Plasmid

   #73215

   Purpose

   Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

   Insert

   EGFR (EGFR Synthetic, Human, Schistosoma japonicum)
   

   Use

   Tags

   10XHis-GST-HRV and The dL5 FAP and Her1 affibody …

   Expression

   Bacterial

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   T7

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Kv7.5 AB (361-545, Δ395-511)/pET28HMT

   Plasmid

   #111267

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.5 AB (361-545, Δ395-511)

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pet28a His6-HSPB7 (zebrafish) C49S/C117S

   Plasmid

   #110073

   Purpose

   Express C49S/C117S double mutant of Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Cysteine 49 to Serine, Cysteine 117 to Serine

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid

    #232116

    Purpose

    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    BCKDK (BCKDK Human)
    

    Use

    Tags

    6 x His, Biotin acceptor peptide (BAP), Maltose-B…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

    Plasmid

    #187356

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192789

    Purpose

    aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody minimizer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. M59 pPET28a-TEV-human beta cat domains R1-C

    Plasmid

    #17200

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    domains R1C

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pET21-10XHis-GST-HRV-Her2-dL5-Her2

    Plasmid

    #85624

    Purpose

    Bacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)

    Depositor

    Marcel Bruchez

    Insert

    10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Human, Schistosoma japonicum, Synthetic)
    

    Use

    Tags

    10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …

    Expression

    Bacterial

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    T7

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-human Beta-catenin R1C (138–781)

    Plasmid

    #132608

    Purpose

    expresses human beta-catenin R1C (138–781) in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

    Insert

    Beta-catenin R1C (138–781)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-10XHis-GST-HRV-Her1-dL5-Her1

    Plasmid

    #73217

    Purpose

    Bacterial expression vector for Z1907-dL5(E52D)-Z1907 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    10XHis-GST-HRV-Her1-dL5-Her1 (EGFR Human, Schistosoma japonicum, Synthetic)
    

    Use

    Tags

    10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …

    Expression

    Bacterial

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    T7

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

    Plasmid

    #229223

    Purpose

    For expression of T. elongatus KaiB protein mutant

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB T. elongatus)
    

    Use

    Tags

    FLAG and SUMO-FLAG

    Expression

    Bacterial

    Mutation

    1-94 (truncated), Y8A, Y94A

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

    Plasmid

    #226349

    Purpose

    Expression of Titin V15P substrate with GS tail

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V15P

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid

    #169135

    Purpose

    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Coh7
    

    Use

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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