We narrowed to 32,111 results for: Eng

1. pAAV-hsyn-GRAB_rDA1m

   Plasmid

   #140556

   Purpose

   Expresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   Red fluorescent DA sensor GRAB_rDA1m (medium affinity)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-5X-TRIM28

   Plasmid

   #45570

   Depositor

   Fanxiu Zhu

   Article

   Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   May 29, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDIRECT_23C

   Plasmid

   #91140

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV5-Flag-HDAC3

   Plasmid

   #63676

   Purpose

   Mammalian expression of Flag tagged HDAC3

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

   Insert

   HDAC3 (Hdac3 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pKH3-TRIM28

   Plasmid

   #45569

   Depositor

   Fanxiu Zhu

   Article

   Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. 6xHsa.NFKB:d2mRFP1

   Plasmid

   #239991

   Purpose

   Destabilized red fluorescent protein (d2mRFP1) under transcriptional control of NF-kB activation

   Depositor

   Raquel Espin-Palazon

   Inserts

   mRFP1
   PEST
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   cfos minimal promoter

   Available since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hsyn-GRAB_DA2h

   Plasmid

   #140554

   Purpose

   Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   The 2nd generation DA sensor GRAB_DA2h (high affinity)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Oct4-IRES-eGFP-PGK-Neo

   Plasmid

   #48681

   Purpose

   For knock-in donor vector by CRISPR/CAS system

   Depositor

   Rudolf Jaenisch

   Article

   Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDIRECT_25H

   Plasmid

   #91145

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ad-Flag-XBP1s

    Plasmid

    #63678

    Purpose

    Adenoviral vector for XBP1s expression

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    spliced

    Promoter

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIRECT_21C

    Plasmid

    #91130

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-TRE-dDIO-vCre

    Plasmid

    #118029

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-dDIO-vCre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE-BR1

    Plasmid

    #160333

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    BR1
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Sc++

    Plasmid

    #155011

    Purpose

    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

    Insert

    Sc++
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T1227K, Loop Substitution from S. anginosus

    Promoter

    EF1ɑ

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-pr-Kaede (Kaede-A69T)

    Plasmid

    #99228

    Purpose

    Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-Kaede
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro

    Plasmid

    #209647

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-Q2L7-T2176N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TRE-vDIO-mScarlet-IRES-tTA

    Plasmid

    #118030

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-vDIO-mScarlet-IRES-tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge