We narrowed to 32,558 results for: Eng

1. pDIRECT_26G

   Plasmid

   #91149

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223249

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM7

   Plasmid

   #201213

   Purpose

   Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.

   Depositor

   Alex Nielsen

   Article

   Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1

   Inserts

   sfGFP
   Spo0A right flank
   Spo0A left flank
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   F11V, T13P, N39D, A179A

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hsyn-GRAB_DA2m

   Plasmid

   #140553

   Purpose

   Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   The 2nd generation DA sensor GRAB_DA2m (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC3.1-caggs-Crimson-CAAX

   Plasmid

   #190041

   Purpose

   Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

   Depositor

   Michael Lin

   Article

   Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

   Insert

   Crimson
   

   Tags

   CAAX

   Expression

   Mammalian

   Promoter

   caggs

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIRECT_22C

   Plasmid

   #91135

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xHsa.NFKB:d2mRFP1

   Plasmid

   #239991

   Purpose

   Destabilized red fluorescent protein (d2mRFP1) under transcriptional control of NF-kB activation

   Depositor

   Raquel Espin-Palazon

   Inserts

   mRFP1
   PEST
   

   Promoter

   cfos minimal promoter

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mm-PylRS-AF/Pyl-tRNACUA

   Plasmid

   #122650

   Purpose

   tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   PylRS-AF, Pyl-tRNACUA
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5-Flag-HDAC3

   Plasmid

   #63676

   Purpose

   Mammalian expression of Flag tagged HDAC3

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

   Insert

   HDAC3 (Hdac3 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-TRE-fDIO-GFP-IRES-tTA

    Plasmid

    #118026

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-fDIO-GFP-IRES-tTA
    

    Use

    AAV

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIRECT_23C

    Plasmid

    #91140

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVTHM-mSox2-3xflag

    Plasmid

    #206376

    Purpose

    Expresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-Puro CAG J591-scFV T-CAR

    Plasmid

    #171961

    Purpose

    Donor plasmid for J591 T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic J591-scFV CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)- e18

    Plasmid

    #131670

    Purpose

    Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-TRIM28

    Plasmid

    #45568

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G34S mutation in EGFP

    Promoter

    CMV

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV-hsyn-GRAB_rDA1h

    Plasmid

    #140557

    Purpose

    Expresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA sensor GRAB_rDA1h (high affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hsyn-GRAB_DA2h

    Plasmid

    #140554

    Purpose

    Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2h (high affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-5X-TRIM28

    Plasmid

    #45570

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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