We narrowed to 74,999 results for: Rest

1. vDip-C: pAAV-Pcp2-mGreenLantern-KASH

   Plasmid

   #207613

   Purpose

   vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

   Depositor

   Longzhi Tan

   Article

   Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   KASH

   Expression

   Mutation

   Promoter

   mouse Pcp2 promoter (L7-6)

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQStrep2-GADD45G

   Plasmid

   #31584

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   growth arrest and DNA-damage-inducible, gamma (GADD45G Human)
   

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3/HIP1/ΔC

   Plasmid

   #31250

   Depositor

   Theodora Ross

   Article

   Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

   Insert

   Huntingtin-interacting protein 1 (HIP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Deltion of nucleotides 1336-2513 1386bp C to T…

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pMY-MCS-P2A-PuroR-HA

   Plasmid

   #163352

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMY-MCS-P2A-CD90.1

   Plasmid

   #163354

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.1 surface marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK-EdnrbE2-Sox10-FLAG-tev-AVI

   Plasmid

   #127775

   Purpose

   Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   sox10 (SOX10 Chicken)
   

   Use

   Enhancer reporter construct

   Tags

   Avi

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Akt1(D323A/D325A)

   Plasmid

   #86630

   Purpose

   Expresses eGFP-tagged human Akt1(D323A/D325A) mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1(D323A/D325A) (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. C25-pLAC

   Plasmid

   #45283

   Purpose

   lacritin-intein fusion plasmid coding for truncated lacritin without signal peptide

   Depositor

   Gordon Laurie

   Article

   Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

   Insert

   pLAC-C25 (LACRT Human)
   

   Use

   Protein expression vector

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   contains aa20-113 of lacritin

   Promoter

   T7

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-LmoCas8b2

   Plasmid

   #126488

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas8b2
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-LmoCas7

    Plasmid

    #126489

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-LmoCas5

    Plasmid

    #126490

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-LmoCas6

    Plasmid

    #126491

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMY-MCS-GSG-eGFP

    Plasmid

    #163350

    Purpose

    Retroviral backbone for in-frame cloning of a C-terminally GFP labeled gene of interest.

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFBD-Akt1(T308A/S473A)

    Plasmid

    #86578

    Purpose

    Expresses human full-length Akt1(T308A/S473A) phosphomutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    T308A/S473A phosphomutant

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe_puro_DEST_Flag_PRKCI

    Plasmid

    #45263

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PKCI (PRKCI Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pHR-FRB:Akt1(D274A)

    Plasmid

    #86634

    Purpose

    Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    FRB-Akt1(D274A) (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lyn-CFP-GAI(1-172)

    Plasmid

    #37313

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Use

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-172

    Promoter

    CMV

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pmCherry-PH1(K14A)

    Plasmid

    #86600

    Purpose

    Expresses mCherry-tagged PH(K14A) domain of human Akt1

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    K14A mutant of Akt1 PH domain (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lyn-CFP-GAI(1-532)

    Plasmid

    #37314

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Use

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-532

    Promoter

    CMV

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only