We narrowed to 25,272 results for: lars

1. nLbCas12a

   Plasmid

   #213745

   Purpose

   For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   nLbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-eLACCO1.1

   Plasmid

   #167944

   Purpose

   Fluorescent biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLACCO1.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEED-mScarlet3:ALFA

   Plasmid

   #219641

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet:ALFA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Nck1

   Plasmid

   #45903

   Depositor

   Louise Larose

   Article

   Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

   Insert

   Nck-1 (NCK1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pET21b-Caspase-3(V266E)

   Plasmid

   #90089

   Purpose

   Expresses caspase 3 V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   V266E

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZW1-FCS-circRNA

   Plasmid

   #73449

   Purpose

   Expression of circular RNA

   Depositor

   Ling-Ling Chen

   Article

   Zhang et al Cell. 2014 Sep 25;159(1):134-47. doi: 10

   Insert

   POLR2A (POLR2A Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV promoter

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSF-CjPglB-LpxE

   Plasmid

   #128389

   Purpose

   Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Inserts

   PglB oligosaccharylktransferase
   LpxE phosphatase
   

   Use

   Tags

   Flag tag

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and pBAD

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV-U6+27-Tornado-Squash-SAM-sensor 5-1

   Plasmid

   #177917

   Purpose

   Expresses Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-Squash-SAM-sensor 5-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LbCas12a-D156R

   Plasmid

   #213744

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-PRMT9

    Plasmid

    #79675

    Purpose

    mammalian expression of GFP-PRMT9

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pHKMC1: Empty sgRNA for Cloning

    Plasmid

    #67720

    Purpose

    Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

    Depositor

    Monica Colaiacovo

    Article

    Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    PU6

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

    Plasmid

    #69906

    Purpose

    Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ciRS7 (CDR1-AS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PU6::unc-119_sgRNA

    Plasmid

    #46169

    Purpose

    unc-119 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    unc-119 targeting sgRNA (unc-119 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-FLEX-HM4Di-YFP

    Plasmid

    #209193

    Purpose

    Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

    Depositor

    Larry Zweifel

    Article

    Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

    Insert

    Hm4Di
    

    Use

    AAV

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CBP-HAT-GST

    Plasmid

    #21093

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    HAT domain: aa 1196-1718

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMB-TMEr

    Plasmid

    #61208

    Purpose

    Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-mCherry-BCP
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP24

    Plasmid

    #70190

    Purpose

    Modular vector for secreted luciferase reporter expression

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

    Insert

    sLucopt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXWJ109Hg-gfp

    Plasmid

    #123148

    Purpose

    A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. YFP-mDia1FH2deltaN

    Plasmid

    #25419

    Depositor

    Arthur Alberts

    Article

    Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

    Insert

    diaph1 (Diaph1 Mouse)
    

    Use

    Tags

    YFP A206K

    Expression

    Mammalian

    Mutation

    mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

    Promoter

    Available since

    July 20, 2010

    Availability

    Academic Institutions and Nonprofits only

20. PU6::klp-12_sgRNA

    Plasmid

    #46170

    Purpose

    klp-12 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    klp-12 targeting sgRNA (klp-12 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge