We narrowed to 11,264 results for: Vars

1. PET-B-eSpCas9-NLS-6xHis

   Plasmid

   #207383

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, amino acids 1005-1013 repl…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ROSA26(MultiFPsΔPuro)

   Plasmid

   #140759

   Purpose

   Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Inserts

   puromycin-N-acetyltransferase
   Venus
   mCherry
   mCerulean
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

   Plasmid

   #218867

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8f
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PH-Btk(R28C)-GFP

   Plasmid

   #51464

   Purpose

   Non-PIP3 binding control for PH-Btk-GFP

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   BTK (aa 1–177) (BTK Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R28C

   Promoter

   CMV

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

5. MAC-BSG

   Plasmid

   #158383

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   BSG (BSG Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1(+)- e18 LOF

   Plasmid

   #131729

   Purpose

   Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ELF3_pet28a

   Plasmid

   #131658

   Purpose

   Bacterial expression of ETS gene ELF3

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELF3 (ELF3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SARS_COV2_ORF3a_HA_BASU

   Plasmid

   #153994

   Purpose

   Express Orf3a gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   Orf3a (ORF3a Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_WDR5_WD

   Plasmid

   #178491

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   WDR5_WD (WDR5 Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-TUBG2

    Plasmid

    #171966

    Purpose

    Expression in cell lines of the TUBG2 gene

    Depositor

    Maria Alvarado-Kristensson

    Article

    Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

    Insert

    TUBG2 (TUBG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Removal of the internal TUBG2-EcoRI site by chang…

    Promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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