We narrowed to 4,714 results for: Drosophila

1. pAC-U63-gRNA2.1

   Plasmid

   #170512

   Purpose

   gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6:3

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBFv-UAS3

   Plasmid

   #138399

   Purpose

   10xUAS expression vector with a vermilion marker and a phiC31 attB sequence

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAS

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-3xP3-RFP

   Plasmid

   #130276

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-RFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-3xP3-GFP

   Plasmid

   #130277

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEED-Dendra2

   Plasmid

   #219645

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Dendra2 SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEED-OLLAS:HA

   Plasmid

   #219647

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   OLLAS:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NCD-GFP

   Plasmid

   #24507

   Depositor

   Ron Vale

   Article

   Case et al Cell. 1997 Sep 5. 90(5):959-66.

   Insert

   NCD (ncd Fly)
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pCRISPaint-sfGFP-3xP3-RFP

   Plasmid

   #127566

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUb RxCas13d + RxCas13d guide RNA

   Plasmid

   #176303

   Purpose

   Expresses RxCas13d and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   RxCas13d
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH3U3-zif268 (omega)

    Plasmid

    #18046

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    zif268 binding site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The preferred binding site for zif268 is inserted…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUb dPspCas13b + PspCas13b guide RNA

    Plasmid

    #176306

    Purpose

    Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    H133A, H1058A

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTV-Gal4DBD2xHighMag1

    Plasmid

    #182023

    Purpose

    Donor plasmid for conversion of Gal4 line into ShineGal4 drivers

    Depositor

    Jean-Paul Vincent

    Article

    di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

    Insert

    Gal4DBD2xHighMag1
    

    Use

    Donor plasmid

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAC-DsRed-QF2-hsp70

    Plasmid

    #104876

    Purpose

    General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    QF2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165887

    Purpose

    G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGE-attBTT-loxP-DsRed (pTL780)

    Plasmid

    #115160

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-TT loxP 3xP3-DsRed
    

    Use

    Cre/Lox

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

    Plasmid

    #71811

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pR6Kg-Alpha1

    Plasmid

    #165862

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJFRC7-UAS-NPRR_ANP

    Plasmid

    #133243

    Purpose

    Fluorescent reporter for neuropeptide release imaging

    Depositor

    David Anderson

    Article

    Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

    Insert

    rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHRW-CRY2olig-VHH(GFP)

    Plasmid

    #126023

    Purpose

    express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CRY2 PHRolig-VHH(GFP)
    

    Use

    Tags

    RFP

    Expression

    Insect

    Mutation

    CRY2 E490G

    Promoter

    Hsp70

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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