We narrowed to 3,145 results for: N-EGFP

1. HLH Bmal Pas 1-465-VenC V435R

   Plasmid

   #47370

   Purpose

   Bmal fragment cloned with Venus tag used for mutation

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Venus aa 156-239

   Expression

   Mammalian

   Mutation

   aa 1-465, V435R

   Promoter

   CMV

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

2. HLH Bmal Pas 1-465-VenC F423R/V435R

   Plasmid

   #47371

   Purpose

   Bmal fragment cloned with Venus tag used for mutation

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Venus aa 156-239

   Expression

   Mammalian

   Mutation

   aa 1-465, F423R/V435R

   Promoter

   CMV

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pRaGE Pyl TAG GFP Y35TAG

   Plasmid

   #160041

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

   Inserts

   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   deGFP
   

   Use

   Synthetic Biology

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed tyrosine 35 to TAG stop-codon (Site numbe…

   Promoter

   P70b promoter and T500 terminator, Vibrio natrieg…

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKIF1C-GFP

   Plasmid

   #130977

   Purpose

   Expression of KIF1C-GFP in mammalian cells.

   Depositor

   Anne Straube

   Article

   Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   CMV

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CIBN-GFP-Sec61B

   Plasmid

   #104177

   Purpose

   Expresses fusion of CIB1 (1-170) with GFP and Sec61B

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CIBN-GFP-Sec61B (CIB1, SEC61B Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAP4K3-GFP d344-873, K45E

   Plasmid

   #21735

   Depositor

   L. Miguel Martins

   Article

   Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

   Insert

   MAP4K3 (MAP4K3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of the carboxy-terminus, amino-acids 344…

   Promoter

   Available Since

   June 3, 2010

   Availability

   Academic Institutions and Nonprofits only

7. GFP-Paxillin DN

   Plasmid

   #186153

   Purpose

   For expressing paxillin variants in mammalian cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PXN (PXN Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Paxilln residues 1-321 added to N-terminus of WT …

   Promoter

   cmv

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS1156

   Plasmid

   #29071

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple39 (CRH Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS1387

   Plasmid

   #29186

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple118 (HTR1A Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1216

    Plasmid

    #29110

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple98 (GPX3 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. V2R untagged

    Plasmid

    #208911

    Purpose

    encodes the human vasopressin receptor 2, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    AVPR2 (AVPR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. V1bR untagged

    Plasmid

    #208925

    Purpose

    encodes the human vasopressin receptor 1b, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    AVPR1B (AVPR1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. anxA2-GFP

    Plasmid

    #107196

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A2 (ANXA2 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Ala residue 65 in human sequence replaced by Glu …

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. alpha actinin delta ABD-GFP

    Plasmid

    #66935

    Purpose

    Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

    Depositor

    Pere Roca-Cusachs, Michael Sheetz

    Article

    Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Alpha-actinin 1 (ACTN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    actin binding domain (amino acids 30–253) is dele…

    Promoter

    CMV

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBactin-Akna-mOrange2

    Plasmid

    #196865

    Purpose

    Expression of the centrosomal protein Akna fused to mOrange2

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akna-mOrange2 (Akna Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmGFP-Sec16S

    Plasmid

    #15775

    Depositor

    Benjamin Glick

    Article

    Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

    Insert

    Sec16S (SEC16B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]

    Plasmid

    #115893

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Has Service

    AAV8

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYO395

    Plasmid

    #235734

    Purpose

    Expression of human VPS34 complex I in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Inserts

    ATG14 (ATG14 Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    

    Use

    Tags

    ZZ-3xTEV

    Expression

    Mammalian

    Mutation

    N256S

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid

    #223106

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid

    #223107

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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