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pAc5.1B-lambdaN-HA-DmTral-LSmTex-del46-49-V5His6_B

Plasmid

#145995

Purpose

Insect Expression of DmTral-LSmTex-del46-49

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmTex-del46-49 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSmTex-del46-49-V5His6_B

Plasmid

#145985

Purpose

Insect Expression of DmTral-LSmTex-del46-49

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmTex-del46-49 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSm-del46-60-V5His6_C

Plasmid

#146068

Purpose

Insect Expression of DmTral-LSm-del46-60

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm-del46-60 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C

Plasmid

#146046

Purpose

Insect Expression of DmTral-LSm-del46-60

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm-del46-60 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_A

Plasmid

#145894

Purpose

Insect Expression of DmCCR4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmCCR4 (twin Fly)

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG1856_A

Plasmid

#145905

Purpose

Insect Expression of CG1856-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

CG1856-3UTR (ttk Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

Plasmid

#146957

Purpose

Insect Expression of DmAGO1-K690A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690A (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-EnR-MCS_N-term

Plasmid

#136884

Purpose

Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-EnR_C-term

Plasmid

#136885

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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mei-S332 5.6Kb genomic DNA in Casper4

Plasmid

#112984

Purpose

P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo) (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 PUM FL

Plasmid

#112997

Purpose

For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

pumilio (pum Fly)

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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UASp-deltaN cycB

Plasmid

#112967

Purpose

P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Lee et al Genetics. 2001 Aug;158(4):1545-56.

Insert

cycB (CycB Fly)

Use

P insertion vector

Mutation

N-term deletion of first 46 amino acids, includes…

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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exon K-renilla

Plasmid

#81007

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Article

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Tags

Renilla luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H04

Plasmid

#70971

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf6 (Taf6 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G05

Plasmid

#70809

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIbeta (CkIIbeta Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B07

Plasmid

#70819

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIalpha (CkIIalpha Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
Hy_pMT Laccase2 MCS-ZKSCAN1 548-847

Plasmid

#69885

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_LRRC31

Plasmid

#45271

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

LRRC31 (LRRC31 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00701

Plasmid

#39737

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6464 (salm Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only