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We narrowed to 7,725 results for: pcr

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Showing: 2861 - 2880 of 7725 results

  1. PASTA-5_pUC19_IVT-ePa01

    Plasmid
    #247041
    Purpose
    Plasmid for in vitro transcription (IVT) of enhanced Pa01 (ePa01) mRNA. Use indicated PCR-primers for correction of T7-mutation and addition of poly-A-tail.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    ePa01 recombinase (large serine integrase, LSR) for IVT
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247041-plasmid-map-sequence-id-500291

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  2. PASTA-1_pUC19_HDR-Bxb1-attG_TRAC_for-Cas12a

    Plasmid
    #247037
    Purpose
    Encodes HDR-template for Cas12a-mediated Knock-In of Bxb1-attG (landing pad) into TRAC gene (exon1). Template region to be PCR-amplified.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    Bxb1 bacterial attachment site (attB) = genomic attachment site (attG), flanked by human TRAC homology arms
    Expression
    Bacterial
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247037-plasmid-map-sequence-id-499187

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  3. pNDGG039

    Plasmid
    #231339
    Purpose
    Terminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Expression
    Bacterial
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231339-plasmid-map-sequence-id-472145

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  4. pNDGG038

    Plasmid
    #231338
    Purpose
    Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Expression
    Bacterial
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231338-plasmid-map-sequence-id-472130

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  5. pNDGG037

    Plasmid
    #231337
    Purpose
    Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Expression
    Bacterial
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231337-plasmid-map-sequence-id-472127

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  8. PASTA-3_pUC19_HDR-Pa01-attG_TRAC_for-Cas12a

    Plasmid
    #247039
    Purpose
    Encodes HDR-template for Cas12a-mediated Knock-In of size-reduced Pa01-attG (landing pad) into TRAC gene (exon1). Template region to be PCR-amplified.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    Pa01 bacterial attachment site (attB) = genomic attachment site (attG), flanked by human TRAC homology arms
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247039-plasmid-map-sequence-id-499186

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  11. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Available since
    Oct. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159752-plasmid-map-sequence-id-313824

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  12. hNTo1-qgRNA-pYJA5

    Plasmid
    #217781
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217781-plasmid-map-sequence-id-466019

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  13. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

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  14. hNTo2-qgRNA-pYJA5

    Plasmid
    #217782
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217782-plasmid-map-sequence-id-466020

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  16. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

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  18. TB202

    Bacterial strain
    #230032
    Purpose
    Fluorescently labelled (mCerulean) E. coli
    Depositor
    Tobias Bergmiller, Calin Guet
    Bacterial resistance
    None
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only

  19. KJ740(DE3)

    Bacterial strain
    #179486
    Purpose
    KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression
    Depositor
    Debra Hansen
    Publication
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Bacterial resistance
    Tetracycline (chromosome, Tn10) (10 μg/mL); Streptomycin/Spectinomycin (chromosome) (50 μg/mL)
    Species
    Escherichia coli
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2861 - 2880 of 7725 results