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pReporter_7

Plasmid

#60567

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_7

Expression

Bacterial

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG S142D

Plasmid

#15939

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S142D

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
pMOS009: pLX304-QuasAr2

Plasmid

#62985

Purpose

Lentivirus production of QuasAr2 fluorescent reporter

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Gateway Cloning and Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Publication

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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FH1-CLGT#2-mCherry-_PGK-H2B-CFP

Plasmid

#164106

Purpose

Fluorescent reporter for genetic tracing of classical Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Publication

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

CLGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNT

Plasmid

#138681

Purpose

Expresses a non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Publication

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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psiCHECK2-E2F1 3'UTR

Plasmid

#29468

Depositor

Judy Lieberman

Publication

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E2F1 3'UTR (E2F1 Human)

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
psiCHECK2-p27Kip1 3'UTR

Plasmid

#29476

Depositor

Judy Lieberman

Publication

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p27 Kip1 3'UTR (CDKN1B Human)

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK2-BRCA1 3'UTR

Plasmid

#29477

Depositor

Judy Lieberman

Publication

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BRCA1 3'UTR (BRCA1 Human)

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
xCas9

Plasmid

#138565

Purpose

Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

xCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Tags

TREER

Expression

Mammalian

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TinyERRE

Plasmid

#208324

Purpose

C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TinyERRE

Tags

TinyERRE

Expression

Mammalian

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PunyEEER

Plasmid

#208326

Purpose

C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PunyEEER

Tags

PunyEEER

Expression

Mammalian

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_485-938_H

Plasmid

#146457

Purpose

Bacterial Expression of NcQDE2_485-938

Depositor

Elisa Izaurralde

Publication

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_485-938

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938_H

Plasmid

#146458

Purpose

Bacterial Expression of NcQDE2_506-938

Depositor

Elisa Izaurralde

Publication

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_506-938

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_517-938_H

Plasmid

#146459

Purpose

Bacterial Expression of NcQDE2_517-938

Depositor

Elisa Izaurralde

Publication

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_517-938

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MvChR1_EYFP_pcDNA3.1

Plasmid

#159119

Purpose

This plasmid encodes MvChR1 rhodopsin domain

Depositor

John Spudich

Publication

Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

Insert

MvChR1

Tags

EYFP

Expression

Mammalian

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-MmUbc7/2776

Plasmid

#11488

Depositor

Allan Weissman

Publication

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MmUbc7 (Ube2g2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

n/a

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only