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mOrange pHis-//

Plasmid

#53304

Purpose

Empty vector to express N-terminal His-mOrange-tagged fusion protein.

Depositor

John Lis

Publication

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Type

Empty backbone

Tags

His and mOrange

Expression

Bacterial

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ908

Plasmid

#44474

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, [4-3] Gateway

Use

Gateway Cloning

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Publication

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-L-R279E

Plasmid

#182286

Purpose

Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Publication

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Expression

Mammalian

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgAMPKa2

Plasmid

#138685

Purpose

Expresses a human AMPKa2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Publication

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa2 human (PRKAA2 Human)

Use

Lentiviral

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

Plasmid

#114068

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for LbCas12a crRNAs

Use

In vitro transcription

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 U6-SaAi9L-U6-SaAi9R

Plasmid

#78604

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

Depositor

Amy Wagers

Publication

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

gRNAs for SaCas9 targeting Ai9 locus

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-N3/hArf6(WT)-EGFP

Plasmid

#79423

Purpose

Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

EGFP

Expression

Mammalian

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(Q67L)-HA

Plasmid

#79425

Purpose

Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 67 to Leucine

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FCK-Chronos-GFP

Plasmid

#80902

Purpose

Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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psiCHECK2-E2F3 3'UTR

Plasmid

#29470

Depositor

Judy Lieberman

Publication

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F3 3'UTR (E2F3 Human)

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pRPR1_c6gRNA_RPR1t

Plasmid

#64384

Purpose

encodes c6 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c6 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_a1gRNA_RPR1t

Plasmid

#64378

Purpose

encodes a1 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

a1 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK3b(Glu, delete LXL)

Plasmid

#50450

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Publication

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted L25-I27, changed Serine 218 to Glutamic A…

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
YCplac22-ENT2

Plasmid

#26684

Depositor

Linda Hicke

Publication

Dores et al Traffic. 2010 Jan . 11(1):151-60.

Insert

ENT2 (ENT2 Budding Yeast)

Expression

Yeast

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pHIS2-hsSTAM1 (1-143)

Plasmid

#21498

Depositor

James Hurley

Publication

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

phis2- STAM1(1-143) (STAM Human)

Tags

6x His

Expression

Bacterial

Mutation

none

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pAGD335

Plasmid

#219499

Purpose

pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K

Expression

Bacterial

Promoter

pBAD

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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MTK2_005

Plasmid

#123700

Purpose

Encodes pCAG as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCAG

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_007

Plasmid

#123702

Purpose

Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pEF1a

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CDK2-3XFlag-pBabe

Plasmid

#73557

Purpose

vector encoding a fusion protein between CDK2 and 3 Flag tags

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

CDK2 (CDK2 Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

SV40

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only