We narrowed to 16,742 results for: Vars

1. pBS7

   Plasmid

   #83774

   Purpose

   Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Venus
   

   Use

   Tags

   Venus

   Expression

   Yeast

   Mutation

   F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SNAP-EF(N)-60nmSAH-GFP(1-10)

   Plasmid

   #61663

   Purpose

   2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   SNAP-EF(N)-60nmSAH-GFP(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-3a-His-MBP-TEV-VC_Cas1_WT

   Plasmid

   #120364

   Purpose

   Expresses wildtype V-C Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VC_Cas1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-3a-His-MBP-TEV-VD_Cas1_WT

   Plasmid

   #120365

   Purpose

   Expresses wildtype V-D Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VD_Cas1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVC-CRISPR1

   Plasmid

   #120366

   Purpose

   Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   CRISPR1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVC-CRISPR

   Plasmid

   #120369

   Purpose

   Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Inserts

   CRISPR1
   CRISPR2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVD_CRISPR

   Plasmid

   #120370

   Purpose

   Plasmid containing shortened version of V-D CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VD_CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLK25

   Plasmid

   #37255

   Depositor

   Ann Hubbard

   Article

   Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

   Insert

   Scribble (SCRIB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 727-1630

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pUAS-MmTFP13xmyc

   Plasmid

   #86371

   Purpose

   membrane targeted Tfp1 expression in zebrafish

   Depositor

   Rachel Wong

   Article

   Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

   Insert

   mTfp1
   

   Use

   Zebrafish

   Tags

   3xMyc and GAP43 membrane targeting

   Expression

   Mutation

   Promoter

   UAS

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. SNAP-EF(N)-5nmSAH-GFP(1-10)

    Plasmid

    #61659

    Purpose

    5nm SAH scaffold with EF Hand and split GFP attachment sites

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    SNAP-EF(N)-5nmSAH-GFP(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLK67

    Plasmid

    #37251

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    Scribble (SCRIB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-728

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pSopBWT

    Plasmid

    #112512

    Purpose

    PsopB driven sopB and sigE

    Depositor

    Olivia Steele-Mortimer

    Article

    Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

    Insert

    sopB/sigE operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsopB

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-oChEF-citrine

    Plasmid

    #50975

    Purpose

    AAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEGFP-30nmSAH-GFP(1-10)

    Plasmid

    #61677

    Purpose

    GFP-labeled SAH scaffold with split GFP attachment site

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    mEGFP-30nmSAH-GFP(1-10)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC57-8xHis

    Plasmid

    #101699

    Purpose

    8xHis in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    AATG-[8xHis]-AGCC
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLK27

    Plasmid

    #37256

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    Scribble (SCRIB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1197-1630

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-6P1-OPNa-delta S

    Plasmid

    #107983

    Purpose

    bacterial expression of full-length OPN

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    OPNa 1080bp-17aa signal (SPP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTagRFP675-actin-C1

    Plasmid

    #44277

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    CMV

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-TRE3G-NODAL-matMYC

    Plasmid

    #115639

    Purpose

    For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330A_FokI-1x4

    Plasmid

    #63604

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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