We narrowed to 6,940 results for: pcdna3.1

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

Plasmid

#163988

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

M1457A

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

Plasmid

#166796

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

KK93EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

Plasmid

#166800

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

E597R

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

Plasmid

#164013

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R464E

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127321

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)

Plasmid

#86818

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Publication

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69896

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-847

Plasmid

#69894

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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NC12 pCDNA3.1 VENUS WTX delta ETGE

Plasmid

#36967

Depositor

Randall Moon

Publication

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX (delta ETGE motif) (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

lacks amino acids 288 –291

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-pri-miR-16-1

Plasmid

#51382

Depositor

Narry Kim

Publication

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

MIR16-1 microRNA 16-1 (MIR16-1 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 V5 hHR23A

Plasmid

#13053

Depositor

Steven Grossman

Publication

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Insert

hHR23A (RAD23A Human)

Tags

His and V5

Expression

Mammalian

Mutation

R325K and L355P relative to NP_001257291.1

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPiezo1-IRES-mScarlet3

Plasmid

#259544

Purpose

Expressing mouse Piezo1 with an mScarlet3 fluorescent reporter.

Depositor

Alexander Chesler

Publication

Nickolls et al Neuron. 2026 Aug 24:S0896-6273(26)00585-

Insert

mPiezo1 (PIEZO1 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPiezo1_N2221K-IRES-mScarlet3

Plasmid

#259545

Purpose

Expressing N2221K-mutant mouse Piezo1 with an mScarlet3 fluorescent reporter.

Depositor

Alexander Chesler

Publication

Nickolls et al Neuron. 2026 Aug 24:S0896-6273(26)00585-

Insert

mPiezo1_N2221K (PIEZO1 Mouse)

Expression

Mammalian

Mutation

N2221K

Promoter

CMV

Available since

Aug. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-)_SARS2_S_WuhanHu1_D614G-H655Y-P681H

Plasmid

#257084

Purpose

Expresses SARS-CoV-2 spike (WuhanHu1 D614G H655Y P681H) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al bioRxiv [Preprint]. 2023 Apr 24:2023.03.

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

D614G, H655Y, P681H

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-)_SARS2_S_WuhanHu1_D614G-H655Y-P681R

Plasmid

#257089

Purpose

Expresses SARS-CoV-2 spike (WuhanHu1 D614G H655Y P681R) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al bioRxiv [Preprint]. 2023 Apr 24:2023.03.

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

D614G, H655Y, P681R

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-GαsBP1-Nluc

Plasmid

#250705

Purpose

Expressing plasma membrane anchored GαsBP1 WT fused to nanoluciferase (Nluc)

Depositor

Mikel Garcia-Marcos

Publication

Zhao et al Nat Chem Biol. 2026 Feb 3. doi: 10.1038/

Insert

mem-G_sBP1 WT-Nluc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-GαsBP2-Nluc

Plasmid

#250704

Purpose

Expressing plasma membrane anchored GαsBP2 WT fused to nanoluciferase (Nluc)

Depositor

Mikel Garcia-Marcos

Publication

Zhao et al Nat Chem Biol. 2026 Feb 3. doi: 10.1038/

Insert

mem-G_sBP2 WT-Nluc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Δ488-592-YFP

Plasmid

#242756

Purpose

E-cadherin 4th domain full deletion

Depositor

Deborah Leckband

Publication

Zou et al J Cell Sci. 2025 Nov 1;138(21):jcs264350

Insert

E-cadherin Δ488-592

Expression

Mammalian

Promoter

CMV

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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