We narrowed to 8,512 results for: sgrna

1. pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2

   Plasmid

   #211688

   Purpose

   U6-sgRNA-EFS-Cas9-P2A-mCherry2

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

   Plasmid

   #194726

   Purpose

   CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   AsCpf1(D908A)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

   Plasmid

   #160570

   Purpose

   A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA:scaffold tetraloop MS2 aptamer SAM
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA targeting human SLC12A5 gene stop codon

   Plasmid

   #158577

   Purpose

   For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   sgRNA targeting human SLC12A5 gene stop codon
   

   Use

   CRISPR

   Tags

   Cas9 and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126758

   Purpose

   Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126759

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126753

   Purpose

   Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126754

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126756

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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