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pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Mouse c-myc (exons II and III)

Plasmid

#8448

Depositor

Frederic Mushinski

Article

Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

Insert

Mouse c-myc (exons II and III) (Myc Mouse)

Expression

Bacterial

Mutation

Exons II and III only

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R61E

Plasmid

#60353

Purpose

R61E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R61E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-N-terminus

Plasmid

#64677

Purpose

expresses only N terminal o WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

Only N terminus amino acids 1-160

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-C-terminus

Plasmid

#64679

Purpose

expresses only C terminus of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

only C terminus amino acids 500-548

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Pur-ottlr3

Plasmid

#225641

Purpose

Expresses the Oncorhynchus tshawytscha toll-like receptor 3

Depositor

Bertrand Collet

Article

Collet et al Mar Biotechnol (NY). 2026 Feb 27;28(2):4

Insert

Toll-like receptor 3 (tlr3 Oncorhynchus tshawytscha)

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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shMYB-2

Plasmid

#247031

Purpose

Used for a knockdown of MYB in human Megakaryocyte/Erythroid Progenitors

Depositor

Diane Krause

Article

Sanada et al Blood. 2016 Aug 18;128(7):923-33. doi: 1

Insert

shMYB-2 (MYB Human)

Use

Lentiviral and RNAi

Promoter

U6

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Tmem2 gRNA

Plasmid

#246569

Purpose

CRISPR vector co-expressing Cas9 and a mouse Tmem2 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Tmem2 gRNA (Cemip2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-H2B-HaloTag-FRT

Plasmid

#247338

Purpose

Expresses H2B-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3HA-Dennd4c

Plasmid

#246276

Purpose

Rab10 GEF

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-Dennd4c (Dennd4c Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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M80

Plasmid

#242632

Purpose

MYC knockdown

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

mCherry (MYC Human)

Use

RNAi

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M74

Plasmid

#242626

Purpose

MYC knockdown

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

MYC (MYC Human)

Use

RNAi

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M56B

Plasmid

#242625

Purpose

Dox-inducible MYC knockdown

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

MYC (MYC Human)

Use

RNAi

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Myc-rDMRdeltaTEAD

Plasmid

#235695

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Myc lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Myc (Myc Mouse)

Use

Luciferase

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Myc-rDMR

Plasmid

#235694

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Myc with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Myc (Myc Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-INO80

Plasmid

#232890

Purpose

Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

INO80 gRNA (INO80 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D683A in active site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521A-peGFP-N1

Plasmid

#228577

Purpose

Expresses mouse TMEM16F-I521A-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I521A plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521E-peGFP-N1

Plasmid

#228578

Purpose

Expresses mouse TMEM16F-I521E-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I521E plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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