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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,661 results for: Tet-on

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Showing: 2901 - 2920 of 7661 results

  7. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

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  14. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid
    #107421
    Purpose
    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    mutation of glutamate 77 to glutamine
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107421-plasmid-map-sequence-id-207622

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  15. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    Maltose-binding protein (MalE)
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107422-plasmid-map-sequence-id-212955

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  17. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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Showing: 2901 - 2920 of 7661 results