We narrowed to 5,303 results for: pcDNA3.1+

1. pcDNA-Ran-Q69L-mRFP1-polyA

   Plasmid

   #104560

   Purpose

   Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Q69L

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. VN-gamma Synuclein

   Plasmid

   #89733

   Purpose

   expresses human gamma Synuclein fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

   Insert

   SNCG (SNCG Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. FlagMBNL3

   Plasmid

   #96901

   Purpose

   expression of Flag-tagged human MBNL3

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   human MBNL3 cDNA (MBNL3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZipGFP11_TEV

   Plasmid

   #81243

   Purpose

   Zipped GFP11 with TEV cleavage sequence

   Depositor

   Xiaokun Shu

   Article

   To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

   Insert

   zipped GFP11 with TEV cleavage sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PalmPalm-ClopHensor

   Plasmid

   #25940

   Depositor

   Daniele Arosio

   Article

   Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

   Insert

   PalmPalm-ClopHensor
   

   Use

   Tags

   Gap20 and Strep-II

   Expression

   Mammalian

   Mutation

   N-term (20AA) of Gap-43

   Promoter

   Available since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRPML2-YFP

   Plasmid

   #18830

   Depositor

   Craig Montell

   Article

   Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

   Insert

   TRPML2 (Mcoln2 Mouse)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-RanWT-mRFP1-polyA

   Plasmid

   #59750

   Purpose

   Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran-mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-Nup37-EGFP-polyA

   Plasmid

   #104562

   Purpose

   Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Nup37 (Nup37 Mouse)
   

   Use

   Tags

   EGFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Plk1 FRET sensor c-jun substrate

   Plasmid

   #45203

   Depositor

   Michael Lampson

   Article

   Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

   Insert

   Plk1 FRET sensor with c-jun substrate
   

   Use

   Tags

   CyPET, YPET

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mVenus-hCOL1A1

    Plasmid

    #140111

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Bongwoori-Pos6

    Plasmid

    #101655

    Purpose

    Bongwoori with the CC1-Pos6 linker length and composition.(* super ecliptic pHluorin A227D)

    Depositor

    Bradley Baker

    Article

    Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

    Insert

    Bongwoori-Pos6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRPML3-HA

    Plasmid

    #18832

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML3 (Mcoln3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

13. mApple-hCOL1A1

    Plasmid

    #140113

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Opto-hEGFR_308

    Plasmid

    #58746

    Purpose

    Expresses Opto-hEGFR (light-activated receptor tyrosine kinase)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    Opto-hEGFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. VinculinTS-A50I

    Plasmid

    #111827

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed alanine 50 to isoleucine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmyc-GFP-TNRC6C

    Plasmid

    #42023

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6C (TNRC6C Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-HIRA-polyA

    Plasmid

    #59781

    Purpose

    Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. VinculinTS-I997A

    Plasmid

    #111828

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed isoleucine 997 to alanine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEQ1180

    Plasmid

    #83805

    Purpose

    express human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells

    Depositor

    Adam Geballe

    Article

    Child et al J Virol. 2012 Apr;86(7):3880-9. doi: 10.

    Insert

    TRS1
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. mV-NcVV-LOV_231

    Plasmid

    #58689

    Purpose

    Expresses mVenus-fusion protein of NcVV-LOV domain (Neurospora crassa Vivid)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    mV-NcVV-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge