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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 22,020 results for: ESC

Showing: 2921 - 2940 of 22020 results

  1. pASPIre3

    Plasmid
    #154844
    Purpose
    Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154844-plasmid-map-sequence-id-307642

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  2. pRSET a MatryoshCaMP6s

    Plasmid
    #100019
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    MatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100019-plasmid-map-sequence-id-194295

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  9. pET15b-eGO-Matryoshka-LE-LP

    Plasmid
    #100017
    Purpose
    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    cpEGFP-LSSmOrange
    Use
    Tags
    6x HIS tag
    Expression
    Bacterial
    Mutation
    Leu and Glu N-term linker and Leu and Pro C-term …
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100017-plasmid-map-sequence-id-194293

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  19. SEP-Nanoluc-cyOFP1

    Plasmid
    #141082
    Purpose
    prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    SEP-Nanoluc-cyOFP1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of amino acids DISGG from positions 673 …
    Promoter
    EF1α
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    141082-plasmid-map-sequence-id-296810

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  20. Arch(D95H)-eGFP

    Plasmid
    #51081
    Purpose
    Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Histidine
    Promoter
    Ubiquitin
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51081-plasmid-map-sequence-id-77349

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Showing: 2921 - 2940 of 22020 results