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Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterxylR-driven promoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB07
Plasmid#190481PurposeTargeting plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsUpstream & Downstream pyrE deletion region
gRNA
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-KRAB-blast
Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABExpressionMutationD10A and H840APromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUV15-pHGFP
Plasmid#70045Purposeencodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cellsDepositorInsertphGFP
UseMycobacterium tuberculosis expression vectorTagsExpressionMutationPromotermycobacterial promoter PsmycAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAM1
Plasmid#60487PurposeThis plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genomeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep
Plasmid#173899PurposeThe pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThrDepositorInsertsThrRS(v1.0)
tRNA(v2.0)CUA
pduX
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJYS2_crtYf
Plasmid#85544PurposeConstitutive transcription of crRNA of crtYf, Spectinomycin resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-CFP
Plasmid#74151PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CeruleanDepositorInsertTol2-kanaR-cryaa-CFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_ALV_Env
Plasmid#207325PurposeExpression of env polyprotein from Avian leukosis virus (ALV)DepositorInsertALV_Env (env Avian leukosis virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.0
Plasmid#86409PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionMutationPromoterAtRPS5AAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE28
Plasmid#90370PurposeTFEB - Lysosomal biogenesis gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterTFEBAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-CMVSP6
Plasmid#138356PurposeGateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)DepositorInsertCMV-SP6 promoter
UseGateway compatibleTagsExpressionMutationPromoterCMV-SP6Available sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMi(3xP3-EGFP)
Plasmid#102540PurposeTransgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP markerDepositorInsertMinos transpoable element with 3xP3-EGFP marker
UseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETa_ mEos4b
Plasmid#51073PurposePhotoconverible Fluorescent Protein_ Fixative Resistant (monomeric)DepositorInsertmEos4b
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE21
Plasmid#90363Purposep53 - Apoptosis gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterp53Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
UseTags6xHis TagExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP64-membrane-mCherry-LOV
Plasmid#135599PurposeExpression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertmembrane-mCherry-LOV
UseIn vitro transcription (mrna synthesis)TagsLOV and mCherryExpressionMutationPromoterUnknownAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA3442
Plasmid#184852PurposeRetroviral vector encoding puromycin resistance and Cre recombinaseDepositorInsertCre
UseCre/Lox and RetroviralTagsMyc-tagExpressionMammalianMutationPromoterLTRAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only