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Plasmid#215165PurposeEncodes the full length of silica binding protein fused at the N-terminus of human metallothioneinDepositorInsertSi-HMT
UseTagsExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Si-FvMT
Plasmid#215166PurposeEncodes the full length of silica binding protein fused at the N-terminus of large algae metallothioneinDepositorInsertSi-FvMT
UseTagsExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Si-ComMT
Plasmid#215167PurposeEncodes the full length of silica binding protein fused at the N-terminus of proteobacteria metallothioneinDepositorInsertSi-ComMT
UseTagsExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pSG5LHARB1sgresistant
Plasmid#229856PurposeRB1DepositorUseTagsHA (N terminal on backbone)ExpressionMammalianMutationchanged a bp24 to g; g bp27 to c, and c bp30 to …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pλPR cI-Elysis
Plasmid#176891PurposeBacterial expression plasmids for production of E. coli bacterial ghost (BG) capsulesDepositorInsertLysis E gene from PhiX174 and lambda PR-cI857 regulatory system
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PDZD8HA_sh_resistant
Plasmid#105006PurposeMammalian Expression of PDZD8, sh resistantDepositorInsertPDZD8 (Pdzd8 Mouse)
UseTagsHAExpressionMammalianMutationThe sequence recognized by shPDZD8 (TRCN000043105…PromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_FLuc_loxP-PuroR
Plasmid#177866PurposeCloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassetteDepositorInsertFLuc-based EXSISERS
UseCRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…TagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag
Plasmid#191572PurposeMutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseTags3x FLAGExpressionMammalianMutationD614G and F486S (confers reduced binding affinity…PromoterCMVAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2
Plasmid#202661PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEXSISERS_NLuc_FRT-PuroR
Plasmid#177865PurposeCloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassetteDepositorInsertNLuc-based EXSISERS
UseCRISPR, Synthetic Biology, and UnspecifiedTagsOLLASExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_BSD_FRT-PuroR
Plasmid#177867PurposeCloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassetteDepositorInsertBSD-based EXSISERS
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsOLLASExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_SurfaceHaloTag_FRT-PuroR
Plasmid#177869PurposeCloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).DepositorInsertSurface-HaloTag-based EXSISERS
UseCRISPR, Synthetic Biology, and UnspecifiedTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SCAF8_isoD_CRISPR_resistant
Plasmid#122468PurposepDONR for Gateway cloningDepositorInsertSCAF8 isoform D CRISPR resistant (SCAF8 Human)
UseGateway cloningTagsExpressionMutationSynonymous mutations conferring resistance to gRN…PromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_0349_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1
Plasmid#116961PurposeExpresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertCellulomonas bogoriensis 69B4 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0350_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1_RC
Plasmid#116962PurposeExpresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertCellulomonas bogoriensis 69B4 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_C1 I3(resist)
Plasmid#61399PurposeExpresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsmCherryExpressionMammalianMutationsilent mutations of bases C183T T192C T189CPromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 RNAi resistant
Plasmid#118347PurposeThis plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationRNAi resistance mutations (does not change amino …PromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only