We narrowed to 4,880 results for: pir

1. pTE4398

   Plasmid

   #74042

   Purpose

   Expresses human codon-optimized LbCpf1 and Lb crRNA.

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Lb crRNA
   LbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-UBC-hAAT-IRES-ZsGreen-W

   Plasmid

   #126702

   Purpose

   lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

   Depositor

   Darrell Kotton

   Article

   Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

   Insert

   Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UBC

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN565

   Plasmid

   #49990

   Purpose

   expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   T7 RNAP*
   

   Use

   Synthetic Biology

   Tags

   umuD degradation tag

   Expression

   Bacterial

   Mutation

   R632S

   Promoter

   pTac-symmetric

   Available since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_PGK_SNIPR_Hinge Notch SNIPR (NRG1 JMD)

   Plasmid

   #188379

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a NRG1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_NRG1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LAMP1-SEP-mRuby3

   Plasmid

   #200939

   Purpose

   endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   LAMP1 (LAMP1 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ230-RRV

   Plasmid

   #188543

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RRV
   

   Use

   CRISPR; Gateway compatible lbcas12a-rrv entry clo…

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, D156R, R182V

   Promoter

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUW-tetO-Myb

   Plasmid

   #139826

   Purpose

   doxycycline-inducible expression of mouse Myb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Myb (Myb Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P94H and Q619R- Please see depositor comment belo…

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUW-tetO-L-MYC

   Plasmid

   #139817

   Purpose

   doxycycline-inducible expression of Human L-MYC in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   L-MYC (MYCL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC5

   Plasmid

   #188602

   Purpose

   Superior Conjugative Plasmid to Diverse Yeast

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC5GGv1

    Plasmid

    #194645

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLGB36

    Plasmid

    #135621

    Purpose

    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

    Depositor

    Laurie Comstock

    Article

    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

    Insert

    bfe3
    

    Use

    Bacteroides suicide vector with inducible counter…

    Tags

    signal sequence for periplasmic targeting

    Expression

    Mutation

    Promoter

    Bacteroides promoter regualted by TetR transcript…

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

    Plasmid

    #160611

    Purpose

    Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:LbCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUW-tetO-NFKB1

    Plasmid

    #139822

    Purpose

    doxycycline-inducible expression of Human NFKB1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF1α:flex-mcherry

    Plasmid

    #222618

    Purpose

    Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

    Depositor

    Franck Polleux

    Article

    Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

    Insert

    Cre-dependent mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. rnLbCas12a-PE2

    Plasmid

    #213746

    Purpose

    For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    rnLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, H759A, R1138A in LbCas12a

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

    Plasmid

    #132408

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dLbaCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R600A; H605A; R1243A; H1248A

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Myc-E-Syt2

    Plasmid

    #66834

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pENN456_TALEcharm_Kv2_MmT8x

    Plasmid

    #220835

    Purpose

    TALEcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    TALEcharm Kv2
    

    Use

    Lentiviral and TALEN

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EF1a

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-LbCpf1-Blast

    Plasmid

    #84751

    Purpose

    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    LbCpf1
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-3xHA (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVRa41_491

    Plasmid

    #49702

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF41_491
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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